BRD7 Antikörper (C-Term)

Produktnummer ABIN7938068

Der Kaninchen Polyklonal anti-BRD7 Antikörper wird verwendet zum Nachweis von BRD7 in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für BRD7 Antikörper (C-Term) (ABIN7938068)

Target: BRD7 (Bromodomain Containing 7 (BRD7))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRD7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-BRD7 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: BRD7 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This BRD7 antibody was raised against a peptide within the C-terminal region of human BRD7.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB*: 1:500 - 1:2,000 dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in 70 mM Tris, 105 mM NaCl, 0.07 mM EDTA, 31 mM glycine, 0.035 % sodium azide, 30 % glycerol, 0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu BRD7

Target: BRD7 (Bromodomain Containing 7 (BRD7))

Andere Bezeichnung: BRD7

Hintergrund:

Background_Plain: BRD7 (Bromodomain-containing protein 7, aka CELTIX1) is a chromatin-associated protein component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 inhibit4ed cancer cell growth and metastasis and promotes apoptosis in vitro and in vivo via downregulating AKT pathway. BRD7 may also regulate signaling pathways like ras-raf-MEK-ERK and RB/E2F. Previous studies have also demonstrated that BRD7 was downregulated in huma breast cancer, and the downregulation is associated with tumor progression.

Aliases: Bromodomain containing 7, CELTIX1, BP75, SMARCI1

Molekulargewicht: 75 kDa

NCBI Accession: NM_013263