Iba1 Antikörper (Intracellular)

Produktnummer ABIN7885099

Der Kaninchen Polyklonal anti-Iba1 Antikörper wird verwendet zum Nachweis von Iba1 in Proben von Ratte und Maus. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für Iba1 Antikörper (Intracellular) (ABIN7885099)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Iba1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Carrier-free

Produktdetails anti-Iba1 Antikörper

Bindungsspezifität: AA 40-53, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to IBA1/AIF1

Homologie: 14 amino acid residues identical,Mouse - 13, human - 11

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DEDLQSKLEAFKTK, corresponding to amino acid residues 40-53 of rat IBA1/AIF1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7234692)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: Ionized calcium-binding adapter molecule 1

Hintergrund:

Synonyms: Ionized calcium-binding adapter molecule 1, Allograft Inflammatory Factor 1, MRF-1, Microglia response factor

Description: Allograft Inflammatory Factor-1 (AIF1) is a 17 kDa. cytoplasmic, calcium and actin binding scaffold protein, mainly expressed in immunocytes. AIF-1 is induced by cytokines such as interferon-γ (IFN-γ).It is encoded within the HLA class III genomic region by IBA1 gene, and its structure contains seven alpha helixes and three beta sheets. There are three other proteins identical to AIF1: Ionized Ca2+-Binding Adaptor 1, Microglia Response Factor-1, and Daintain1.The gene is highly expressed in testis, spleen, circulating macrophages and in the brain but weakly expressed in lung and kidney. Among brain cells, the Iba1 gene is strongly and specifically expressed in microglia and its expression is up-regulated following nerve injury, central nervous system ischemia, and several other brain diseases2.AIF1 influences the immune system at several key points and thus modulates inflammatory diseases. It increases the expression of inflammatory mediators such as cytokines, chemokines, inducible nitric oxide synthase (iNOS) and promotes inflammatory cell proliferation and migration and hence it is highly associated with autoimmune diseases3. In addition, its abundance is positively correlated with metabolic indicators, such as body mass index, triglycerides, and fasting plasma glucose levels. There is also evidence that AIF1 could be a marker for diabetic nephropathy when detected in serum. It is found in activated macrophages in the pancreatic islets, and has been shown to decrease insulin secretion, while simultaneously impairing glucose elimination4.

Gen-ID: 29427

UniProt: P55009

Pathways: Smooth Muscle Cell Migration