Gephyrin Antikörper (Intracellular)

Produktnummer ABIN7885038

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Gephyrin in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für Gephyrin Antikörper (Intracellular) (ABIN7885038)

Target: Gephyrin (GPHN)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Gephyrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Carrier-free

Produktdetails anti-Gephyrin Antikörper

Bindungsspezifität: AA 183-195, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Gephyrin

Homologie: Mouse,human - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235588)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Gephyrin

Target: Gephyrin (GPHN)

Andere Bezeichnung: GPHN

Hintergrund:

Synonyms: GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase

Description: Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR), it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.

Gen-ID: 64845

UniProt: Q03555

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development