GABARAP Antikörper (Intracellular, N-Term)

Produktnummer ABIN7885007

Der Kaninchen Polyklonal anti-GABARAP Antikörper (ABIN7885007) detektiert spezifisch GABARAP in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für GABARAP Antikörper (Intracellular, N-Term) (ABIN7885007)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABARAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Produktdetails anti-GABARAP Antikörper

Bindungsspezifität: AA 2-15, Intracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to GABA Receptor Associated Protein

Homologie: Mouse,human - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KFVYKEEHPFEKRR, corresponding to amino acid residues 2-15 of rat GABARAP

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:800

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235469)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Andere Bezeichnung: GABA receptor associated protein

Hintergrund:

Synonyms: GABA receptor associated protein, GABARAPL

Description: GABA(A) receptors are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABA associated protein (GABARAP) family interacts specifically with the γ1, γ2S and γ2L subunits of the GABA(A) receptor. GABARAP is expressed in a variety of tissues including heart, brain, placenta, liver and others. The genetic sequence encoding GABARAP is similar to microtubule-associated proteins 1A and 1B1.GABARAP has an asymmetric structure with a basic N-terminal region and an acidic C terminus. GABARAP has a compact fold consisting of four or five-stranded β-sheets with two α-helices on each side. The outer strands of the β-sheets are aligned anti-parallel to the inner strands and helices α3 and α4 are located on one side of the sheet. The presence of α1 and α2 is a unique feature of GABARAP and forms a hydrophilic patch suitable for protein-protein interface. GABARAP exists in a monomeric conformation at low protein concentration and a head-to-tail oligomeric conformation in high salt conditions.GABARAP is involved in intracellular trafficking and is an integral part of the intra-Golgi transport machinery. Thus it is detected in cellular compartments such as the Golgi apparatus and endoplasmic reticulum. GABARAP affects GABA(A) receptors' cell surface expression by increasing their intracellular trafficking. Disruption of cellular microtubules prevents the clustering of both GABARAP and GABA(A) receptors. GABARAP also interacts with other receptors such as the glycine receptor at the postsynaptic site2.

Gen-ID: 58974

UniProt: P60517

Pathways: Autophagie