GPR65 Antikörper (Extracellular Loop)

Produktnummer ABIN7884992

Der Kaninchen Polyklonal anti-GPR65 Antikörper (ABIN7884992) detektiert spezifisch GPR65 in IHC, FACS, LCI, WB und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für GPR65 Antikörper (Extracellular Loop) (ABIN7884992)

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR65 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI), Western Blotting (WB), Immunofluorescence (IF)

Güteklasse: Carrier-free

Produktdetails anti-GPR65 Antikörper

Bindungsspezifität: AA 72-84, Extracellular Loop

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR65 (TDAG8)

Homologie: Mouse - 12,13 amino acid residues identical, rat - 11,13 amino acid residues identical.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:1200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235434)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR65

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Andere Bezeichnung: G-protein coupled receptor 65

Hintergrund:

Synonyms: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor

Description: GPR65, also called TDAG8 (T-cell death associated gene 8 protein), is a pH -sensing G-protein coupled receptor (GPCR) activated by acidic extracellular pH through the protonation of several histidine residues in the receptor's sequence. GPR65 is involved in cancer cell metastasis and proliferation, immune cell function, inflammation, and blood vessel formation1.Human GPR65 gene has been mapped to a location that is associated with T cell lymphoma and leukemia abnormalities.GPR65 is primarily expressed in immune cells and leukocyte-rich tissues such as circulating peripheral leukocytes, spleen, thymus, and tonsils. Expression is also detected pain relevant loci such as the dorsal root ganglia neurons and particularly small diameter neurons responsible for nociception1,2.GPR65 has the ability to affect tumor development and growth. Overexpression of GPR65 in Lewis lung carcinoma cells increases tumor growth in mice and may facilitate resistance to acidosis-mediated cell death in vitro through protein kinase A (PKA) and ERK related pathways1.Knockdown of GPR65 in NCI-H460 human non-small cell lung cancer cells decreases cell survival in acidic condition. Another study shows that activation of GPR65 by acidosis can promote evasion of cell apoptosis under glutamine starvation and its overexpression has been reported to transform immortalized mammary epithelial cells1.

Gen-ID: 8477

UniProt: Q8IYL9

Pathways: cAMP Metabolic Process