GPR65 Antikörper (Extracellular Loop)

Produktnummer ABIN7884991

Der Kaninchen Polyklonal anti-GPR65 Antikörper wird verwendet zum Nachweis von GPR65 in Proben von Human, Maus und Ratte. Er wurde validiert für FACS, LCI und WB.

Kurzübersicht für GPR65 Antikörper (Extracellular Loop) (ABIN7884991)

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR65 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Live Cell Imaging (LCI), Western Blotting (WB)

Güteklasse: Carrier-free

Produktdetails anti-GPR65 Antikörper

Bindungsspezifität: AA 72-84, Extracellular Loop

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR68 (OGR1)

Homologie: Rat,mouse,human - 14,15 amino acid residues identical.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235434)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR65

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Andere Bezeichnung: G-protein coupled receptor 65

Hintergrund:

Synonyms: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Description: All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54 % ) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.

Gen-ID: 238377

UniProt: Q8BFQ3

Pathways: cAMP Metabolic Process