Cortactin Antikörper (Intracellular)

Produktnummer ABIN7884899

Der Kaninchen Polyklonal anti-Cortactin Antikörper (ABIN7884899) detektiert spezifisch Cortactin in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Cortactin Antikörper (Intracellular) (ABIN7884899)

Target: Cortactin (CTTN)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cortactin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Produktdetails anti-Cortactin Antikörper

Bindungsspezifität: AA 346-359, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Cortactin

Homologie: Rat,human - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KTSNIRANFENLAK, corresponding to amino acid residues 346 - 359 of mouse Cortactin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:1000

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235169)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Cortactin

Target: Cortactin (CTTN)

Andere Bezeichnung: Src substrate cortactin

Hintergrund:

Synonyms: Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN

Description: Cortical acting binding protein, Cortactin, is an actin-binding protein involved in cytoskeletal regulation1. Cortactin is a 63-65 kDa protein that consists of four major domains: N-terminal acidic (NTA), 6.5 tandems repeat, proline-rich domain, and the C-terminal Src homology 3 domains (SH3 domains)2 and it is regulated by post translational modifications such as phosphorylation and acetylation1.The actin filament network provides mechanical support as well as protrusive and contractile forces essential for directed cell motility and cell and tissue morphogenesis. Cortactin activates the Arp2/3 complex, the main regulator of actin polymerization, and thereby regulates the assembly of branched actin3.Overexpression of cortactin was shown to enhance cell migration, invasion, and tumor cell metastasis. It is also overexpressed in various types of cancer such as breast cancers as well as head and neck tumors1.

Gen-ID: 13043

UniProt: Q60598

Pathways: MAPK Signalweg