N-Cadherin Antikörper (Intracellular)

Produktnummer ABIN7884805

Der Kaninchen Polyklonal anti-N-Cadherin Antikörper wird verwendet zum Nachweis von N-Cadherin in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC.

Kurzübersicht für N-Cadherin Antikörper (Intracellular) (ABIN7884805)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser N-Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Produktdetails anti-N-Cadherin Antikörper

Bindungsspezifität: AA 794-807, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to N-Cadherin

Homologie: Rat,human - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794-807 of mouse CDH2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7234896)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: Cadherin 2

Hintergrund:

Synonyms: Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal

Description: Cadherins are transmembrane proteins responsible for cell adhesion. Cell adhesion is a crucial feature for the sustainability and maintenance of normal tissue function and three dimensional structure. Cadherins share an extracellular domain consisting of multiple repeats of cadherin-specific motif and are calcium dependent homotypic cell-to-cell adhesion molecules. They are localized mostly in specialized sites called adherence junctions1.N-cadherin consists of an amino-terminal external domain with five tandem repeats, a single transmembrane segment, and a cytoplasmic carboxy-terminal domain of approximately 150 amino acids. The intracellular domain of N-cadherin interacts with a group of proteins called catenins that are essential for cadherin-mediated cell adhesion. It is suggested that N-cadherin proteins align in a form of "zipper" when involved in cell adhesion. Cadherins on one cell surface form a series of rigid dimers that attach to equivalent dimers on the opposing cells and lateral motion of these complexes allows the cell junction site to "zip up"1.N-cadherin plays a role in tumor metastasis. Unlike E-cadherin that causes tumor metastasis when insufficiently, N-cadherin is involved in tumor metastasis when upregulated. N-cadherin stimulates migration, invasion and metastasis of squamous cell breast cancer and its effects are increased by FGF-2 suggesting that N-cadherin and FGFR-1 synergize and promote aggressive tumor behavior. This synergy also promotes neuronal growth1. Interestingly, low levels of N-cadherin are associated with a higher invasive capacity of astrocytic glioma by increasing tumor (and normal) cell migration speed and creating a less organized pattern of migration3.

Gen-ID: 12558

UniProt: P15116

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane