Rekombinanter NFE2 Antikörper

Produktnummer ABIN7881833

Der Kaninchen Monoklonal anti-NFE2 Antikörper (Klon 31N33) (ABIN7881833) detektiert spezifisch NFE2 in WB, IHC, IF, IP und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter NFE2 Antikörper (ABIN7881833)

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser NFE2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 31N33

Produktdetails anti-NFE2 Antikörper

Verwendungszweck: NFE2 Antibody / Nuclear factor erythroid 2

Aufreinigung: Affinity chromatography

Immunogen: A synthesized peptide derived from human NFE2 was used as the immunogen for the NFE2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NFE2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the NFE2 antibody at -20oC.

Details zu NFE2

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Andere Bezeichnung: NFE2

Hintergrund: NFE2 antibody targets Nuclear factor erythroid 2, a transcription factor encoded by the NFE2 gene. NFE2 belongs to the basic leucine zipper family and is composed of a heterodimer of a large DNA-binding subunit (p45) and a small subunit from the Maf protein family. This transcription factor plays a central role in the regulation of erythroid and megakaryocytic cell differentiation. NFE2 antibody provides an essential reagent for studying gene expression programs controlling hematopoiesis, oxidative stress responses, and platelet production.

The p45 subunit of NFE2 binds to antioxidant response elements and regulates transcription of genes involved in heme biosynthesis, hemoglobin assembly, and detoxification of reactive oxygen species. In megakaryocytes, NFE2 is required for proplatelet formation and platelet release. Detection of this factor using NFE2 antibody helps identify regulatory pathways that maintain normal blood development and function.

Mutations or dysregulation of NFE2 are implicated in hematological disorders, including myeloproliferative neoplasms. Overexpression of NFE2 has been reported in polycythemia vera and essential thrombocythemia, linking the transcription factor to abnormal erythrocyte and platelet production. Research employing NFE2 antibody has revealed roles in leukemogenesis and abnormal megakaryopoiesis. Because of these connections, NFE2 is studied as a potential therapeutic target in blood cancers.

NFE2 antibody can be applied in western blotting, immunohistochemistry, and electrophoretic mobility shift assays. Western blotting confirms protein expression in erythroid cell lines, while immunohistochemistry highlights tissue-specific nuclear localization. In gene regulation studies, chromatin immunoprecipitation with NFE2 antibody allows mapping of target promoters controlled by this transcription factor.

NFE2 is also relevant in oxidative stress research. Its regulation of antioxidant genes links it to cellular defense mechanisms against free radicals and environmental toxins. Researchers using NFE2 antibody can explore how transcriptional control intersects with oxidative signaling and stress adaptation. NSJ Bioreagents supports hematology and transcription factor research by providing validated antibodies against NFE2.

UniProt: Q16621