NFE2 Antikörper (Center)

Produktnummer ABIN2855460

Produktdetails anti-NFE2 Antikörper

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Bindungsspezifität: Center

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFE2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Kreuzreaktivität: Affe, Schwein

Kreuzreaktivität (Details): Monkey (97 %), Pig (91 %)

Produktmerkmale: Rabbit Polyclonal antibody to NF-E2 (nuclear factor (erythroid-derived 2), 45 kDa)
NF-E2 antibody [N1C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NF-E2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation Assay-dependent dilution Western blot 1:500-1:3000* ChIP-sequencing Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ImmunoprecipitationAssay-dependent dilution Western blot1:500-1:3000* ChIP-sequencingAssay-dependent dilution

Kommentare:

Positive Control: 293T , mouse spleen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.03 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu NFE2

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Andere Bezeichnung: NF-E2 (NFE2 Produkte)

Synonyme: NF-E2 antikoerper, p45 antikoerper, 45kDa antikoerper, p45NFE2 antikoerper, p45nf-e2 antikoerper, nuclear factor, erythroid 2 antikoerper, nuclear factor, erythroid derived 2 antikoerper, NFE2 antikoerper, Nfe2 antikoerper

Hintergrund: Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Cellular Localization: Nucleus

Molekulargewicht: 41 kDa

Gen-ID: 4778