CD45RA Antikörper

Produktnummer ABIN7880312

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD45RA in IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CD45RA Antikörper (ABIN7880312)

Target: CD45RA

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45RA Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM568

Produktdetails anti-CD45RA Antikörper

Verwendungszweck: Anti-CD45RA Antibody

Aufreinigung: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as immunogen for this anti-CD45RA antibody.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Due to variation in protocol and secondary antibody used, the anti-CD45RA antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The anti-CD45RA antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Details zu CD45RA

Target: CD45RA

Andere Bezeichnung: CD45RA

Hintergrund: CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ T-cells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

UniProt: P08575