CD45RA Antikörper (CD45RA)

Details for Product anti-CD45RA Antibody No. ABIN94136
Antigen
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Lca
  • RT7
  • protein tyrosine phosphatase, receptor type C
  • protein tyrosine phosphatase, receptor type, C
  • PTPRC
  • Ptprc
Reaktivität
Human
354
19
10
7
5
2
1
1
Wirt
Maus
386
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD45RA Antikörper ist unkonjugiert
50
49
25
22
12
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA), Western Blotting (WB)
363
153
107
89
82
43
43
29
6
6
4
3
1
Optionen
Immunogen Human thymocytes and T lymphocytes.
Klon MEM-56
Isotyp IgG2b
Spezifität The antibody MEM-56 reacts with CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.
HLDA IV, WS Code NL 907
Kreuzreaktivität (Details) Human
Reinigung Purified by protein-A affinity chromatography
Reinheit > 95 % (by SDS-PAGE)
Antigen
Andere Bezeichnung CD45RA (CD45RA Antibody Abstract)
Hintergrund CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Western Blotting (WB) image for anti-CD45RA (CD45RA) antibody (ABIN94136) Western Blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymph...
Allgemeine Veröffentlichungen Dawes, Petrova, Liu, Wraith, Beverley, Tchilian: "Combinations of CD45 isoforms are crucial for immune function and disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3417-25, 2006 (PubMed).

Cosenza-Nashat, Kim, Zhao, Suh, Lee: "CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis." in: Brain pathology (Zurich, Switzerland), Vol. 16, Issue 4, pp. 256-65, 2006 (PubMed).

Li, Tsuyama, Ishikawa, Obata, Abroun, Liu, Otsuyama, Zheng, Ma, Maki, Kawano: "A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma." in: Blood, Vol. 105, Issue 8, pp. 3295-302, 2005 (PubMed).

Produkt verwendet in: Bazil, Stefanová, Hilgert, Kristofová, Van?k, Bukovský, Horejsí: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, 1990 (PubMed).