Caldesmon Antikörper

Produktnummer ABIN7880139

Der Maus Monoklonal anti-Caldesmon Antikörper wird verwendet zum Nachweis von Caldesmon in Proben von Human. Er wurde validiert für IHC (p).

Kurzübersicht für Caldesmon Antikörper (ABIN7880139)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caldesmon Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM168

Produktdetails anti-Caldesmon Antikörper

Verwendungszweck: Caldesmon Antibody / CALD1 / CAD (HMW)

Aufreinigung: Protein G affinity chromatography

Immunogen: Crude human uterus extract was used as the immunogen for the Caldesmon antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Caldesmon antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Caldesmon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Andere Bezeichnung: Caldesmon

Hintergrund: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction