Rekombinanter DPYSL2 Antikörper (pThr514)

Produktnummer ABIN7879800

Der Kaninchen Monoklonal anti-DPYSL2 Antikörper wird verwendet zum Nachweis von DPYSL2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für Rekombinanter DPYSL2 Antikörper (pThr514) (ABIN7879800)

Target: DPYSL2 (Dihydropyrimidinase-Like 2 (DPYSL2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser DPYSL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 32D10

Produktdetails anti-DPYSL2 Antikörper

Bindungsspezifität: pThr514

Verwendungszweck: Phospho-CRMP2 (Thr514) Antibody / Collapsin response mediator protein 2

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-CRMP2 (T514) was used as the immunogen for the Phospho-CRMP2 (Thr514) antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Phospho-CRMP2 (Thr514) antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the Phospho-CRMP2 (Thr514) antibody at -20oC.

Details zu DPYSL2

Target: DPYSL2 (Dihydropyrimidinase-Like 2 (DPYSL2))

Andere Bezeichnung: CRMP2

Hintergrund: Phospho-CRMP2 (Thr514) antibody detects collapsin response mediator protein 2 when phosphorylated at threonine 514. CRMP2, encoded by the DPYSL2 gene, is a cytosolic phosphoprotein that regulates axon guidance, neuronal polarity, and microtubule dynamics. Phosphorylation at Thr514, primarily mediated by GSK3 beta, reduces CRMP2 binding to tubulin and destabilizes microtubules. This modification plays critical roles in neuronal development and synaptic remodeling.

Phospho-CRMP2 (Thr514) antibody is widely applied in neuroscience research, particularly in studies of axon guidance and neurodegeneration. By detecting phosphorylated CRMP2, researchers can distinguish between active and inhibited states of the protein. Elevated phosphorylation of CRMP2 at Thr514 has been associated with Alzheimer disease and other neurodegenerative conditions where microtubule stability is compromised.

The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho-CRMP2 (Thr514) antibody detects distinct phosphorylated isoforms. Immunohistochemistry highlights tissue specific phosphorylation patterns in brain regions, while immunofluorescence enables visualization of phospho CRMP2 at growth cones and synapses. These applications make the antibody valuable for developmental neuroscience and disease studies.

Phosphorylation of CRMP2 at Thr514 regulates not only axonal outgrowth but also dendritic spine morphology and synaptic plasticity. Dysregulation of this site contributes to cognitive decline and neuropsychiatric disorders. By applying Phospho-CRMP2 (Thr514) antibody, scientists can explore mechanisms linking abnormal phosphorylation to neuronal dysfunction and identify therapeutic approaches that target CRMP2 signaling.

Phospho-CRMP2 (Thr514) antibody from NSJ Bioreagents provides reliable specificity for detecting phosphorylated DPYSL2, enabling accurate monitoring of microtubule regulatory pathways in health and disease.

UniProt: Q16555

Pathways: Regulation of Cell Size