Rekombinanter PKC Antikörper (pan)

Produktnummer ABIN7879699

Der Kaninchen Monoklonal anti-PKC Antikörper (Klon 32P76) (ABIN7879699) detektiert spezifisch PKC in WB, IF, IHC, IP, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Rekombinanter PKC Antikörper (pan) (ABIN7879699)

Target: PKC (Protein Kinase C (PKC))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PKC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 32P76

Produktdetails anti-PKC Antikörper

Bindungsspezifität: pan

Verwendungszweck: Pan-PKC Antibody / PRKCA/PRKCB/PRKCZ

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PKC was used as the immunogen for the Pan-PKC antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Pan-PKC antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the Pan-PKC antibody at -20oC.

Details zu PKC

Target: PKC (Protein Kinase C (PKC))

Andere Bezeichnung: PKC

Hintergrund: Pan-PKC antibody detects protein kinase C isoforms alpha, beta, and zeta, encoded by the PRKCA, PRKCB, and PRKCZ genes. PKC proteins are a family of serine/threonine kinases activated by diacylglycerol, calcium, and phospholipids. They regulate diverse processes including proliferation, apoptosis, migration, and secretion. The PKC family is divided into conventional, novel, and atypical subgroups based on activation requirements. PKC alpha belongs to the conventional subgroup requiring both calcium and diacylglycerol, PKC beta has two splice variants beta I and beta II, while PKC zeta is atypical and functions independently of calcium and diacylglycerol. By detecting multiple isoforms simultaneously, Pan-PKC antibody provides a broad tool for studying PKC biology.

Pan-PKC antibody is widely applied in cancer biology, immunology, and neuroscience. PKC alpha regulates proliferation and apoptosis in many cell types, PKC beta is critical for B cell receptor signaling, and PKC zeta participates in NF-kappaB signaling and polarity regulation. Together, these isoforms integrate signaling from growth factors, immune receptors, and stress pathways. By applying Pan-PKC antibody, researchers can examine global PKC expression and activity across diverse systems.

Applications for Pan-PKC antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect multiple PKC isoforms in cell and tissue lysates, immunohistochemistry maps distribution in tumors and immune organs, and immunofluorescence reveals subcellular localization at membranes, cytoskeleton, and nuclei. These approaches allow characterization of PKC biology at multiple levels.

Dysregulation of PKC isoforms contributes to cancer, autoimmunity, diabetes, and cardiovascular disease. Overactivation of PKC promotes tumor growth, invasion, and angiogenesis, while loss of function impairs immune signaling and metabolic regulation. By detecting PKC isoforms broadly, Pan-PKC antibody supports research into how kinase networks contribute to disease. In leukemia, aberrant PKC activity modulates drug sensitivity, while in diabetes, PKC activation contributes to vascular complications.

PKC isoforms also play roles in neuronal signaling, where they regulate neurotransmitter release and plasticity. By applying Pan-PKC antibody, scientists can study PKC activity in synaptic function and memory. Therapeutically, PKC inhibitors are under investigation for oncology and metabolic disorders. Monitoring PKC expression with antibody-based assays provides biomarkers for drug development and clinical research.

NSJ Bioreagents provides Pan-PKC antibody with validated specificity for multiple isoforms. Its performance supports reliable detection of PKC alpha, beta, and zeta across cancer, immunology, and neuroscience applications.

UniProt: Q05513

Pathways: T-Zell Rezeptor Signalweg, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Phototransduction