FUBP1 Antikörper (N-Term)

Produktnummer ABIN7879600

Der Kaninchen Polyklonal anti-FUBP1 Antikörper (ABIN7879600) detektiert spezifisch FUBP1 in WB, IHC, IF und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für FUBP1 Antikörper (N-Term) (ABIN7879600)

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FUBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-FUBP1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: FUBP1 Antibody / Far upstream element binding protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FUBP1 was used as the immunogen for the FUBP1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the FUBP1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the FUBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu FUBP1

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Andere Bezeichnung: FUBP1

Hintergrund: FUBP1 antibody detects Far upstream element binding protein 1, encoded by the FUBP1 gene on chromosome 1p31.1. FUBP1 antibody is widely used to investigate this single-stranded DNA- and RNA-binding protein that regulates transcription, mRNA stability, and translation. FUBP1 belongs to the FUBP family, which also includes FUBP2 and FUBP3, and was first identified as a regulator of c-Myc by binding the far upstream element (FUSE) located upstream of the MYC promoter. By acting as a transcriptional activator or repressor depending on cellular context, FUBP1 plays a central role in controlling cell proliferation, apoptosis, and differentiation.

Structurally, FUBP1 contains multiple KH (hnRNP K homology) domains that mediate binding to single-stranded nucleic acids. These domains allow recognition of pyrimidine-rich elements in DNA and RNA, enabling FUBP1 to regulate gene transcription and mRNA metabolism. Its nuclear localization sequences direct it to the nucleus, where it interacts with transcriptional machinery, while cytoplasmic pools of FUBP1 influence mRNA turnover and translation. This versatility positions FUBP1 as a bridge between transcriptional and post-transcriptional control.

Functionally, FUBP1 is a key regulator of c-Myc, one of the most important proto-oncogenes. It stimulates transcription by binding the FUSE region and recruiting cofactors that promote RNA polymerase II elongation. FUBP1 also regulates genes involved in apoptosis, metabolism, and cell cycle progression. In addition, FUBP1 modulates mRNA stability by interacting with AU-rich elements and influences translation of transcripts involved in development and stress responses. Researchers use FUBP1 antibody to probe these diverse functions in molecular and cancer biology.

Clinically, FUBP1 mutations and altered expression are linked to multiple cancers. Deletions of FUBP1 are common in oligodendrogliomas, where they contribute to tumor development alongside IDH1 mutations and 1p/19q codeletion. In contrast, overexpression of FUBP1 supports tumor progression in hepatocellular carcinoma, leukemia, and other malignancies by sustaining c-Myc activity. Beyond cancer, FUBP1 has been implicated in hematopoiesis, neuronal development, and metabolic regulation. Dysregulated expression is associated with developmental delay and intellectual disability syndromes. These findings underscore the importance of FUBP1 as both a biomarker and potential therapeutic target.

Experimentally, FUBP1 antibody is used in western blotting to detect the ~68 kDa protein, in immunohistochemistry to evaluate tumor samples, and in immunofluorescence to visualize its nuclear distribution. Co-immunoprecipitation with FUBP1 antibody has identified interactions with transcriptional regulators including TFIIH and p300. Studies also demonstrate interactions with splicing factors and RNA-binding proteins, linking transcriptional and post-transcriptional networks. NSJ Bioreagents supplies FUBP1 antibody to support research in oncology, gene regulation, and RNA biology.

UniProt: Q96AE4