Far Upstream Element (FUSE) Binding Protein 1 (FUBP1) (AA 540-589) Antikörper

Details zu Produkt Nr. ABIN6741561
Antigen
Epitop
AA 540-589
10
9
7
7
3
3
2
2
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Human, Affe, Schwein, Kaninchen, Ratte (Rattus)
77
38
31
7
6
5
5
4
3
2
2
2
2
1
Wirt
Kaninchen
60
11
7
Klonalität
Polyklonal
Konjugat
Unkonjugiert
6
4
3
3
3
3
2
2
2
2
2
2
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
65
39
16
10
7
5
5
4
2
2
1
1
Optionen
Marke IHC-plus™
Immunogen Synthetic peptide located between aa540-589 of human FUBP1 (Q96AE4, NP_003893). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Rat, Elephant, Bovine, Rabbit, Pig (100%), Gorilla, Dog (93%), Mouse, Guinea pig, Stickleback (92%), Galago (87%), Turkey, Chicken, Platypus (85%), Bat (81%).

Type of Immunogen: Synthetic peptide
Isotyp IgG
Spezifität Human FUBP1
Homologie Percent identity by BLAST analysis: Human, Rat, Bovine, Rabbit (100%) Dog (93%) Mouse, Guinea pig (92%) Chicken (85%).
Reinigung Immunoaffinity purified
Antigen
Andere Bezeichnung FUBP1 (FUBP1 Antibody Abstract)
Hintergrund Name/Gene ID: FUBP1

Synonyms: FUBP1, DNA helicase V, FBP, HDH V, FUSE-binding protein 1, FUBP
Gen-ID 8880
NCBI Accession NP_003893
UniProt Q96AE4
Applikations-hinweise Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Kommentare

Target Species of Antibody: Human

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Distilled water
Konzentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handhabung Avoid repeat freeze-thaw cycles.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Bilder des Herstellers
Image no. 1 for anti-Far Upstream Element (FUSE) Binding Protein 1 (FUBP1) (AA 540-589) antibody (ABIN6741561) Human Breast (formalin-fixed, paraffin-embedded) stained with FUBP1 antibody ABIN4621...
Image no. 2 for anti-Far Upstream Element (FUSE) Binding Protein 1 (FUBP1) (AA 540-589) antibody (ABIN6741561) Human Colon (formalin-fixed, paraffin-embedded) stained with FUBP1 antibody ABIN46219...