PARL Antikörper (Middle Region)

Produktnummer ABIN7879543

Der Kaninchen Polyklonal anti-PARL Antikörper (ABIN7879543) detektiert spezifisch PARL in WB und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für PARL Antikörper (Middle Region) (ABIN7879543)

Target: PARL (Presenilin Associated, Rhomboid-Like (PARL))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PARL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-PARL Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: PARL Antibody / Presenilins-associated rhomboid-like protein

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PARL was used as the immunogen for the PARL antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PARL antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PARL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PARL

Target: PARL (Presenilin Associated, Rhomboid-Like (PARL))

Andere Bezeichnung: PARL

Hintergrund: PARL antibody detects Presenilins-associated rhomboid-like protein, a mitochondrial intramembrane protease involved in regulating mitochondrial dynamics, apoptosis, and mitophagy. The UniProt recommended name is Presenilins-associated rhomboid-like protein (PARL). This serine protease participates in the regulated intramembrane proteolysis of substrates that control mitochondrial morphology and quality control.

Functionally, PARL antibody identifies a 379-amino-acid protein localized to the inner mitochondrial membrane. PARL cleaves and activates key mitochondrial substrates such as PINK1 and PGAM5, initiating mitophagy and apoptosis signaling pathways. Through these actions, PARL maintains mitochondrial homeostasis and protects cells from oxidative stress. It also participates in remodeling the inner membrane during stress conditions, influencing cristae organization and mitochondrial fission-fusion balance.

The PARL gene is located on chromosome 3q27.1 and is expressed in metabolically active tissues including brain, heart, and skeletal muscle. Expression levels fluctuate with energy demand and stress, with upregulation observed during mitochondrial biogenesis and differentiation. PARL is evolutionarily conserved across eukaryotes, emphasizing its fundamental role in organelle maintenance.

Pathologically, mutations in PARL have been associated with mitochondrial dysfunction, Parkinson's disease, and metabolic disorders. Impaired PARL activity disrupts PINK1 processing, leading to defective mitophagy and accumulation of damaged mitochondria. In contrast, overexpression may alter apoptotic sensitivity by influencing cytochrome c release. Research using PARL antibody supports studies in mitochondrial biology, apoptosis regulation, and neurodegenerative disease.

PARL antibody can be validated for western blotting, immunofluorescence, and immunohistochemistry to detect mitochondrial proteases. NSJ Bioreagents provides PARL antibody reagents optimized for research in mitophagy, proteolytic signaling, and mitochondrial quality control.

Structurally, Presenilins-associated rhomboid-like protein contains seven transmembrane domains forming a proteolytic chamber within the inner mitochondrial membrane. Its catalytic serine and histidine residues form the rhomboid protease active site. The protein's C-terminal region undergoes regulated processing, modulating its activity in stress conditions. This antibody enables investigation of PARL's role in mitochondrial proteostasis and apoptosis signaling.

UniProt: Q9H300

Pathways: Autophagie