PABPN1 Antikörper (Middle Region)

Produktnummer ABIN7879541

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PABPN1 in WB, IF, IHC, IP, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für PABPN1 Antikörper (Middle Region) (ABIN7879541)

Target: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PABPN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-PABPN1 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: PABPN1 Antibody / Polyadenylate-binding protein 2

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PABPN1 was used as the immunogen for the PABPN1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PABPN1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PABPN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PABPN1

Target: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Andere Bezeichnung: PABPN1

Hintergrund: PABPN1 antibody detects Polyadenylate-binding protein 2, encoded by the PABPN1 gene. Polyadenylate-binding protein 2 is a nuclear protein that binds to the poly(A) tail of RNA and regulates mRNA processing, stability, and translation. PABPN1 antibody provides researchers with a critical reagent for studying RNA metabolism, gene expression, and human disease associated with RNA dysregulation.

Polyadenylate-binding protein 2 is essential for the regulation of poly(A) tail length and stability of nuclear transcripts. Research using PABPN1 antibody has shown that it stimulates poly(A) polymerase activity and ensures that poly(A) tails reach optimal length for nuclear export and translation. By binding RNA, PABPN1 prevents premature deadenylation and degradation, thereby safeguarding mRNA function.

Studies with PABPN1 antibody have revealed that mutations in PABPN1 cause oculopharyngeal muscular dystrophy, a late-onset muscle disorder characterized by progressive weakness of eyelid, pharyngeal, and proximal limb muscles. The disease results from short expansions of a GCN trinucleotide repeat in the gene, which cause protein aggregation and dysfunction. This clinical connection underscores the importance of PABPN1 in human health.

Beyond muscular dystrophy, research using PABPN1 antibody has shown that the protein regulates alternative polyadenylation, influencing transcript diversity and gene expression patterns. Dysregulation of polyadenylation contributes to cancer, neurodegeneration, and developmental disorders, making PABPN1 a key factor in RNA biology.

PABPN1 antibody is widely applied in western blotting, immunohistochemistry, and RNA immunoprecipitation. Western blotting quantifies protein levels in muscle and brain tissue, immunohistochemistry demonstrates nuclear localization, and RNA immunoprecipitation identifies target transcripts. These applications make PABPN1 antibody indispensable for RNA processing research.

By providing validated PABPN1 antibody reagents, NSJ Bioreagents supports studies into polyadenylation, muscular dystrophy, and RNA regulation. Detection of Polyadenylate-binding protein 2 provides researchers with insight into how nuclear RNA-binding proteins regulate transcript stability and disease.

UniProt: Q86U42