CD24 Antikörper (Middle Region)

Produktnummer ABIN7879510

Der Kaninchen Polyklonal anti-CD24 Antikörper (ABIN7879510) detektiert spezifisch CD24 in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CD24 Antikörper (Middle Region) (ABIN7879510)

Target: CD24 (CD24 Molecule (CD24))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD24 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CD24 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: CD24 Antibody

Aufreinigung: Antigen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CD24 protein was used as the immunogen for the CD24 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CD24 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the CD24 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu CD24

Target: CD24 (CD24 Molecule (CD24))

Andere Bezeichnung: CD24

Hintergrund: CD24 antibody detects Cluster of differentiation 24, a small glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein involved in cell adhesion, immune modulation, and cancer metastasis. Encoded by the CD24 gene on chromosome 6q21, this protein is expressed on developing hematopoietic cells, mature B cells, granulocytes, and various epithelial and neuronal tissues. CD24 mediates cell-cell and cell-matrix interactions by binding to P-selectin and Siglec family receptors, influencing immune cell trafficking and signaling.

Structurally, CD24 is a heavily glycosylated protein lacking a transmembrane domain, anchored to the plasma membrane via a GPI linkage. The carbohydrate moieties of CD24 contribute to its interactions with lectins and adhesion molecules, which regulate lymphocyte homing and activation. In the nervous system, CD24 is involved in axon growth and synaptic plasticity, while in the immune system it acts as a costimulatory molecule modulating T cell responses. CD24 engagement can influence B cell maturation, dendritic cell activation, and apoptosis signaling.

The CD24 antibody is widely used in immunology, oncology, and stem cell biology research to study immune signaling, cancer progression, and differentiation. Flow cytometry using this antibody identifies CD24-positive cell populations, while western blot analysis detects the mature glycoprotein at approximately 35-40 kilodaltons, with size variability due to glycosylation. Immunohistochemistry reveals membrane staining in lymphoid tissues and various carcinomas.

In cancer research, CD24 has emerged as a key marker of tumor-initiating cells and metastasis. Overexpression is associated with aggressive tumor phenotypes in breast, ovarian, and colorectal cancers, where CD24 promotes cell adhesion to P-selectin on platelets and endothelial cells, aiding metastatic spread. Conversely, CD24 expression can modulate immune responses by interacting with Siglec-10, inhibiting innate immune activation. The CD24 antibody is an essential tool for investigating immune regulation, tumor biology, and cell surface glycoprotein signaling. NSJ Bioreagents provides this antibody validated for immunohistochemistry, ensuring high specificity in cellular and molecular studies.

UniProt: P25063

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation