LIG1 Antikörper (full length)

Produktnummer ABIN7878534

Der Maus Monoklonal anti-LIG1 Antikörper wird verwendet zum Nachweis von LIG1 in Proben von Human, Ratte und Maus. Er wurde validiert für WB und ELISA.

Kurzübersicht für LIG1 Antikörper (full length) (ABIN7878534)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LIG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Güteklasse: Carrier-free

Klon: 1A9

Produktdetails anti-LIG1 Antikörper

Bindungsspezifität: full length

Verwendungszweck: DNA Ligase 1 Antibody / LIG1 (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: Full-length native cow LIG1 protein was used as the immunogen for the DNA Ligase 1 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the DNA Ligase 1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the DNA Ligase 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Andere Bezeichnung: DNA Ligase 1

Hintergrund: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

UniProt: P18858

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA