NUPR1 Antikörper (C-Term)

Produktnummer ABIN7877439

Der Kaninchen Polyklonal anti-NUPR1 Antikörper wird verwendet zum Nachweis von NUPR1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF, FACS und ICC.

Kurzübersicht für NUPR1 Antikörper (C-Term) (ABIN7877439)

Target: NUPR1 (Nuclear Protein, Transcriptional Regulator, 1 (NUPR1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUPR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-NUPR1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: NUPR1 Antibody / Nuclear protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NUPR1 was used as the immunogen for the NUPR1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NUPR1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NUPR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NUPR1

Target: NUPR1 (Nuclear Protein, Transcriptional Regulator, 1 (NUPR1))

Andere Bezeichnung: NUPR1

Hintergrund: NUPR1 antibody detects Nuclear protein 1, a small chromatin-associated transcriptional regulator induced by cellular stress. Encoded by the NUPR1 gene on chromosome 16p11.2, this intrinsically disordered nuclear protein is activated in response to stress stimuli such as oxidative damage, hypoxia, and nutrient deprivation. NUPR1 modulates gene expression related to cell cycle control, apoptosis, and autophagy, playing a pivotal role in stress adaptation and tumor progression.

Structurally, NUPR1 is a 100-amino-acid protein of approximately 9 kilodaltons lacking defined secondary structure, which enables flexible interactions with DNA and chromatin-modifying enzymes. It acts as a transcriptional cofactor rather than a classical DNA-binding protein, partnering with p300, SMADs, and histone modifiers to influence transcriptional responses. NUPR1 localizes predominantly to the nucleus but can shuttle between compartments depending on stress conditions and post-translational modifications.

The NUPR1 antibody is widely used in oncology, stress biology, and transcription research to study adaptive gene regulation and tumor cell survival. Western blot analysis detects a 9 kilodalton band corresponding to NUPR1, while immunofluorescence demonstrates strong nuclear localization that increases following stress induction. This antibody enables analysis of NUPR1 expression during oxidative and metabolic stress and its contribution to stress-responsive transcription networks.

Functionally, NUPR1 promotes survival under cytotoxic conditions by regulating genes involved in autophagy and anti-apoptotic pathways. It acts downstream of ATF4 and CHOP in the endoplasmic reticulum stress response and influences cell fate decisions by modulating p53-dependent transcription. In cancer, overexpression of NUPR1 supports tumor growth, resistance to chemotherapy, and epithelial-mesenchymal transition (EMT). Conversely, NUPR1 depletion sensitizes tumor cells to oxidative damage and metabolic inhibitors. The NUPR1 antibody provides a key research tool for exploring stress signaling, transcriptional plasticity, and tumor adaptability. NSJ Bioreagents validates this antibody for its applications, ensuring sensitive and reproducible detection across experimental systems.

UniProt: O60356