HSPA6 Antikörper (C-Term)

Produktnummer ABIN7877394

Der Kaninchen Polyklonal anti-HSPA6 Antikörper wird verwendet zum Nachweis von HSPA6 in Proben von Human. Er wurde validiert für WB und FACS.

Kurzübersicht für HSPA6 Antikörper (C-Term) (ABIN7877394)

Target: HSPA6 (Heat Shock 70kDa Protein 6 (HSP70B') (HSPA6))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPA6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-HSPA6 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: HSPA6 Antibody / Heat shock 70 kDa protein 6

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA6 was used as the immunogen for the HSPA6 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the HSPA6 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the HSPA6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu HSPA6

Target: HSPA6 (Heat Shock 70kDa Protein 6 (HSP70B') (HSPA6))

Andere Bezeichnung: HSPA6

Hintergrund: HSPA6 antibody detects Heat shock 70 kDa protein 6, a stress-inducible molecular chaperone that belongs to the HSP70 family of heat shock proteins. The UniProt recommended name is Heat shock 70 kDa protein 6 (HSPA6). This protein is rapidly upregulated in response to heat shock, oxidative stress, and heavy metal exposure, where it assists in refolding denatured proteins and protecting cells from proteotoxic damage.

Functionally, HSPA6 antibody identifies a 646-amino-acid cytoplasmic and nuclear chaperone that binds unfolded or misfolded polypeptides to prevent aggregation and promote refolding. HSPA6 operates through an ATP-dependent mechanism, alternating between substrate-binding and release cycles coordinated by its nucleotide-binding and substrate-binding domains. Unlike constitutive HSP70 family members, HSPA6 expression is strictly inducible and transient, making it a sensitive marker of acute cellular stress. It cooperates with co-chaperones such as HSP40 and nucleotide exchange factors to restore proteostasis during recovery from stress.

The HSPA6 gene is located on chromosome 1q23.3 and is transcriptionally regulated by heat shock factor 1 (HSF1). Expression is strongly induced by thermal stress and other cytotoxic insults in epithelial, neuronal, and endothelial cells. Under normal physiological conditions, HSPA6 levels are low or undetectable, but during stress, it rapidly accumulates in both the cytoplasm and nucleus, where it stabilizes denatured proteins and facilitates their refolding or degradation.

Pathologically, HSPA6 has been implicated in neuroprotection, cardiac stress response, and cancer cell survival. Upregulation in neurons protects against protein misfolding diseases such as Alzheimer's and Parkinson's, while elevated expression in tumors may contribute to resistance against apoptosis and chemotherapy. In cardiac tissues, HSPA6 supports survival under ischemic or oxidative stress conditions. Research using HSPA6 antibody supports studies in proteostasis, cellular stress pathways, and molecular chaperone biology.

HSPA6 antibody is validated for use in relevant research applications to detect inducible heat shock proteins and examine cellular responses to thermal or oxidative stress. NSJ Bioreagents provides HSPA6 antibody reagents optimized for studies in protein folding, stress signaling, and chaperone-mediated cytoprotection.

UniProt: P17066