CRH Antikörper (C-Term)

Produktnummer ABIN7877355

Der Kaninchen Polyklonal anti-CRH Antikörper wird verwendet zum Nachweis von CRH in Proben von Human, Ratte und Maus. Er wurde validiert für WB und FACS.

Kurzübersicht für CRH Antikörper (C-Term) (ABIN7877355)

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CRH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-CRH Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: CRH Antibody / Corticotropin Releasing Hormone

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CRF/CRH was used as the immunogen for the CRH antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CRH antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the CRH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Andere Bezeichnung: CRH

Hintergrund: CRH antibody detects Corticotropin releasing hormone, a neuropeptide hormone that regulates the hypothalamic-pituitary-adrenal (HPA) axis and stress response. The UniProt recommended name is Corticotropin releasing hormone (CRH). This 41-amino-acid peptide is synthesized in the hypothalamus and secreted into the hypophyseal portal circulation, where it stimulates adrenocorticotropic hormone (ACTH) release from the anterior pituitary.

Functionally, CRH antibody identifies the peptide precursor prepro-CRH, which undergoes proteolytic cleavage to produce the mature active hormone. CRH binds to G protein-coupled receptors CRHR1 and CRHR2 on pituitary corticotrophs, activating adenylate cyclase and cAMP-dependent signaling pathways that induce ACTH synthesis and secretion. ACTH in turn stimulates cortisol release from the adrenal cortex, completing the neuroendocrine feedback loop that maintains stress adaptation and homeostasis.

The CRH gene is located on chromosome 8q13.1 and is primarily expressed in the paraventricular nucleus of the hypothalamus, but also in peripheral tissues such as the placenta, adrenal glands, and gastrointestinal tract. CRH coordinates endocrine, autonomic, and behavioral responses to stress, linking neuroendocrine signaling with emotional and metabolic regulation.

Pathologically, dysregulated CRH expression is associated with anxiety, depression, Cushingi 1/2s syndrome, and inflammatory disorders. Overproduction of CRH leads to chronic activation of the HPA axis and elevated cortisol levels, while deficiency impairs stress response and adrenal function. Research using CRH antibody supports studies in neuroendocrinology, stress physiology, and behavioral neuroscience.

CRH antibody is validated for immunohistochemistry, ELISA, and immunofluorescence to detect neuropeptides and hormones. NSJ Bioreagents provides CRH antibody reagents optimized for research in endocrine signaling, stress regulation, and hypothalamic function.

Structurally, Corticotropin releasing hormone is a 41-residue peptide containing a disulfide bond that stabilizes its helical structure, essential for receptor binding and biological activity. Its precursor includes a signal peptide directing secretion and prohormone regions involved in processing. This antibody enables investigation of CRH's role in HPA axis regulation, neuropeptide signaling, and stress-related disorders.

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour