PARP16 Antikörper (AA 9-254)

Produktnummer ABIN7877069

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PARP16 in FACS, IHC und ELISA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PARP16 Antikörper (AA 9-254) (ABIN7877069)

Target: PARP16 (Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PARP16 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Produktdetails anti-PARP16 Antikörper

Bindungsspezifität: AA 9-254

Verwendungszweck: PARP16 Antibody / Poly ADP-ribose polymerase 16

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human PARP16 recombinant protein (Position: A9-Y254) was used as the immunogen for the PARP16 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PARP16 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PARP16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PARP16

Target: PARP16 (Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16))

Andere Bezeichnung: PARP16

Hintergrund: PARP16 antibody detects Poly(ADP-ribose) polymerase 16, a membrane-associated mono-ADP-ribosyltransferase that regulates the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress signaling. PARP16 belongs to the PARP family of enzymes involved in ADP-ribosylation, a post-translational modification that controls protein stability and signaling. The PARP16 antibody is commonly used in studies of ER stress, apoptosis, and ADP-ribosylation-dependent signaling pathways.

PARP16 is encoded by the PARP16 gene located on human chromosome 15q21.2. The protein is approximately 38 kilodaltons and uniquely localized to the ER membrane, where it functions as a tail-anchored enzyme with its catalytic domain facing the cytoplasm. Unlike canonical PARPs that catalyze poly(ADP-ribosylation), PARP16 primarily transfers single ADP-ribose moieties to substrate proteins (mono-ADP-ribosylation). This modification modulates the activity of key ER stress sensors, including PERK and IRE1-alpha, enhancing their signaling output during the UPR.

The PARP16 antibody typically detects a 38 kilodalton protein by western blot, and immunofluorescence confirms its ER localization. Functional studies demonstrate that PARP16 activity is induced upon ER stress caused by misfolded protein accumulation or chemical perturbation. Activated PARP16 promotes phosphorylation and activation of PERK and IRE1-alpha, leading to downstream transcriptional responses that restore proteostasis. Inhibition or depletion of PARP16 compromises the UPR, resulting in increased sensitivity to ER stress and enhanced apoptosis.

Beyond its role in ER stress regulation, PARP16 contributes to cancer cell survival and resistance to chemotherapeutic agents that induce proteotoxic stress. Overexpression has been reported in hepatocellular carcinoma, breast cancer, and melanoma, where it supports adaptive stress signaling. Conversely, loss of PARP16 sensitizes cells to stress-induced death, making it a potential therapeutic target. NSJ Bioreagents provides a validated PARP16 antibody optimized for immunofluorescence, immunohistochemistry and flow cytometry assays, enabling precise study of ADP-ribosylation mechanisms and ER homeostasis.

UniProt: Q8N5Y8