DVL2 Antikörper (AA 89-576)

Produktnummer ABIN7877061

Der Kaninchen Polyklonal anti-DVL2 Antikörper wird verwendet zum Nachweis von DVL2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für DVL2 Antikörper (AA 89-576) (ABIN7877061)

Target: DVL2 (Dishevelled, Dsh Homolog 2 (Drosophila) (DVL2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DVL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-DVL2 Antikörper

Bindungsspezifität: AA 89-576

Verwendungszweck: DVL2 Antibody / Disheveled segment polarity protein 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human DVL2 recombinant protein (Position: L89-Y576) was used as the immunogen for the DVL2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the DVL2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the DVL2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu DVL2

Target: DVL2 (Dishevelled, Dsh Homolog 2 (Drosophila) (DVL2))

Andere Bezeichnung: DVL2

Hintergrund: DVL2 antibody detects Disheveled segment polarity protein 2, a cytoplasmic phosphoprotein that serves as a key scaffold in canonical and non-canonical Wnt signaling pathways. Encoded by the DVL2 gene on chromosome 17p13.1, this protein belongs to the Disheveled family, which transduces Wnt receptor activation into downstream signaling controlling cell polarity, differentiation, and proliferation. DVL2 contains DIX, PDZ, and DEP domains that mediate interactions with Frizzled receptors, Axin, and downstream kinases. These domains enable DVL2 to coordinate beta-catenin stabilization in the canonical pathway and planar cell polarity signaling in non-canonical branches.

Upon Wnt ligand binding, DVL2 is recruited to the membrane through Frizzled receptors, where it inhibits the beta-catenin destruction complex composed of Axin, APC, and GSK-3beta. This allows beta-catenin to accumulate and enter the nucleus, where it activates Wnt target genes that regulate growth and differentiation. In addition to its role in the canonical Wnt pathway, DVL2 participates in planar cell polarity signaling, influencing cytoskeletal organization and tissue morphogenesis. Dysregulation of DVL2 expression or phosphorylation disrupts normal Wnt signaling and has been implicated in developmental disorders and multiple cancers, including breast, colon, and lung carcinoma.

The DVL2 antibody is widely used in cell signaling, developmental biology, and oncology to monitor Wnt pathway activation and Disheveled protein dynamics. Western blot analysis identifies multiple DVL2 bands (90-100 kilodaltons) corresponding to phosphorylated isoforms, while immunofluorescence reveals punctate cytoplasmic and membrane-associated staining. This antibody supports investigation of Wnt-mediated transcriptional regulation and cytoskeletal rearrangement. Because DVL2 serves as a convergence point for canonical and non-canonical pathways, it is crucial for studying cell polarity, migration, and tissue patterning.

Beyond development, DVL2 influences cancer progression, stem cell renewal, and inflammation through its regulation of beta-catenin and JNK signaling. NSJ Bioreagents provides the DVL2 antibody validated for its applications, ensuring high specificity in research on Wnt signaling and tumor biology.

UniProt: O14641

Pathways: Tube Formation