NUP85 Antikörper (AA 84-656)

Produktnummer ABIN7876962

Der Kaninchen Polyklonal anti-NUP85 Antikörper wird verwendet zum Nachweis von NUP85 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, ICC, IF und FACS.

Kurzübersicht für NUP85 Antikörper (AA 84-656) (ABIN7876962)

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUP85 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-NUP85 Antikörper

Bindungsspezifität: AA 84-656

Verwendungszweck: NUP85 Antibody / Nuclear pore complex protein Nup85

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NUP85 recombinant protein (Position: R84-S656) was used as the immunogen for the NUP85 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NUP85 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NUP85 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NUP85

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Andere Bezeichnung: NUP85

Hintergrund: NUP85 antibody detects Nuclear pore complex protein Nup85, encoded by the NUP85 gene on chromosome 17q11.2. NUP85 antibody is used to study this nucleoporin, a stable component of the nuclear pore complex (NPC) that mediates bidirectional transport of macromolecules between the nucleus and cytoplasm. NUP85 forms part of the NUP107-160 subcomplex, which assembles on both sides of the NPC and is critical for pore biogenesis, nuclear envelope formation, and transport selectivity. NPCs are essential for cell viability, controlling import of transcription factors, RNA-binding proteins, and nuclear enzymes while exporting mRNAs, snRNAs, and ribosomal subunits.

Structurally, NUP85 is a beta-propeller protein containing helical domains that scaffold the NUP107-160 subcomplex. It interacts directly with NUP107, NUP133, and NUP160 to form a stable assembly platform. Cryo-electron microscopy studies have mapped NUP85 within the NPC ring, where it contributes to pore architecture and nucleocytoplasmic transport dynamics. In addition to transport, NUP85 participates in chromatin organization and gene expression, linking the nuclear periphery to regulatory networks.

Functionally, NUP85 is indispensable for cell cycle progression. During mitosis, NPCs disassemble and reassemble, and the NUP107-160 complex drives nuclear envelope reformation. Loss of NUP85 disrupts pore assembly, causing nuclear transport defects and cell cycle arrest. In higher eukaryotes, NUP85 also contributes to developmental programs by influencing signaling pathways such as MAPK and Wnt, mediated through nuclear import of transcriptional regulators. NUP85 antibody is a powerful reagent for dissecting these processes.

Clinically, mutations in NUP85 are associated with nephrotic syndrome and steroid-resistant kidney disease. These phenotypes reflect impaired podocyte nuclear transport and altered gene expression. NUP85 has also been linked to cancer biology, where dysregulated nucleocytoplasmic transport promotes proliferation and therapy resistance. Studies reveal NUP85 overexpression in glioblastoma and breast cancer, suggesting a role in tumor progression. By applying NUP85 antibody, researchers can analyze NPC integrity, nuclear transport activity, and disease mechanisms. NSJ Bioreagents provides NUP85 antibody for use in cell biology, nephrology, and oncology research.

UniProt: Q9BW27