Rekombinanter Cytochrome C Antikörper (AA 81-104, AA 93-104)

Produktnummer ABIN7876877

Dieser Maus Monoklonal Antikörper detektiert spezifisch Cytochrome C in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für Rekombinanter Cytochrome C Antikörper (AA 81-104, AA 93-104) (ABIN7876877)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: RCYCS-1010

Produktdetails anti-Cytochrome C Antikörper

Bindungsspezifität: AA 81-104, AA 93-104

Verwendungszweck: Recombinant Cytochrome C Antibody (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: A human partial protein corresponding to amino acids 81-104 of pigeon Cytochrome C was used as the immunogen for this recombinant Cytochrome C antibody. The epitope has been localized to amino acids 93-104.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the recombinant Cytochrome C antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant Cytochrome C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: Cytochrome C

Hintergrund: In mammalian cells, the highly conserved cytochrome C protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).

UniProt: P99999

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity