Rekombinanter Geminin Antikörper (AA 71-202)

Produktnummer ABIN7876603

Der Kaninchen Monoklonal anti-Geminin Antikörper (Klon GMNN-7037R) (ABIN7876603) detektiert spezifisch Geminin in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter Geminin Antikörper (AA 71-202) (ABIN7876603)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Geminin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: GMNN-7037R

Produktdetails anti-Geminin Antikörper

Bindungsspezifität: AA 71-202

Verwendungszweck: Recombinant Geminin Antibody / GMNN

Aufreinigung: Protein A/G affinity

Immunogen: A portion of amino acids 71-202 was used as the immunogen for the recombinant Geminin antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the recombinant Geminin antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the recombinant Geminin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Details zu Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Andere Bezeichnung: Geminin

Hintergrund: Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication