Geminin Antikörper (AA 71-202)

Produktnummer ABIN7876600

Der Maus Monoklonal anti-Geminin Antikörper (Klon GMNN-4033) (ABIN7876600) detektiert spezifisch Geminin in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Geminin Antikörper (AA 71-202) (ABIN7876600)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Geminin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: GMNN-4033

Produktdetails anti-Geminin Antikörper

Bindungsspezifität: AA 71-202

Verwendungszweck: GMNN Antibody / Geminin (azide and preservative free)

Aufreinigung: Protein A/G affinity

Immunogen: A portion of amino acids 71-202 was used as the immunogen for the GMNN antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the GMNN antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the GMNN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Details zu Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Andere Bezeichnung: GMNN

Hintergrund: Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication