HJURP Antikörper (AA 6-594)

Produktnummer ABIN7875970

Der Kaninchen Polyklonal anti-HJURP Antikörper (ABIN7875970) detektiert spezifisch HJURP in WB, ELISA, FACS, IF und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für HJURP Antikörper (AA 6-594) (ABIN7875970)

Target: HJURP (Holliday Junction Recognition Protein (HJURP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HJURP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-HJURP Antikörper

Bindungsspezifität: AA 6-594

Verwendungszweck: HJURP Antibody / Holliday junction recognition protein

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human HJURP recombinant protein (Position: R6-K594) was used as the immunogen for the HJURP antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the HJURP antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the HJURP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu HJURP

Target: HJURP (Holliday Junction Recognition Protein (HJURP))

Andere Bezeichnung: HJURP

Hintergrund: HJURP antibody detects Holliday junction recognition protein, a histone chaperone responsible for depositing the centromere-specific histone H3 variant CENP-A at centromeres during DNA replication. Encoded by the HJURP gene on chromosome 2q37.1, this protein is essential for centromere identity and chromosome segregation fidelity. HJURP functions as a dedicated assembly factor that ensures proper incorporation of newly synthesized CENP-A into centromeric nucleosomes during the cell cycle.

Structurally, HJURP is a 748-amino-acid nuclear protein of approximately 80 kilodaltons containing an N-terminal Scm3-like domain that binds CENP-A/H4 heterodimers and a C-terminal domain responsible for centromere targeting through interaction with Mis18 complex components. HJURP localizes exclusively to centromeres during late telophase and early G1 phase, coordinating with the Mis18alpha/beta complex to mediate chromatin assembly and maintain centromere identity.

The HJURP antibody is widely used in cell cycle, chromatin assembly, and cancer research to study centromere formation, histone variant deposition, and chromosomal stability. Western blot analysis detects an 80 kilodalton band corresponding to HJURP, while immunofluorescence reveals discrete nuclear foci at centromeric regions. This antibody provides a key tool for analyzing chromosome segregation fidelity and CENP-A deposition dynamics.

Functionally, HJURP acts as the exclusive chaperone for CENP-A nucleosome assembly, ensuring the maintenance of centromeric chromatin identity across cell divisions. Loss or depletion of HJURP leads to chromosome mis-segregation, aneuploidy, and genomic instability, hallmark features of tumorigenesis. Elevated HJURP expression has been reported in various cancers and correlates with poor prognosis due to its role in promoting proliferation and mitotic progression. The HJURP antibody supports studies on chromatin inheritance, mitotic regulation, and epigenetic maintenance of centromeres. NSJ Bioreagents validates this antibody for its applications, providing a dependable reagent for centromere biology and mitotic research.

UniProt: Q8NCD3