ATG7 Antikörper (AA 540-569)

Produktnummer ABIN7875662

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ATG7 in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für ATG7 Antikörper (AA 540-569) (ABIN7875662)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ATG7 Antikörper

Bindungsspezifität: AA 540-569

Verwendungszweck: ATG7 Antibody / APG7

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 540-569 from the human protein was used as the immunogen for the ATG7 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the ATG7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Details zu ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Andere Bezeichnung: ATG7

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagie