NETO1 Antikörper (AA 5-514)

Produktnummer ABIN7875312

Der Kaninchen Polyklonal anti-NETO1 Antikörper wird verwendet zum Nachweis von NETO1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für NETO1 Antikörper (AA 5-514) (ABIN7875312)

Target: NETO1 (Neuropilin (NRP) and Tolloid (TLL)-Like 1 (NETO1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NETO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-NETO1 Antikörper

Bindungsspezifität: AA 5-514

Verwendungszweck: NETO1 Antibody / Neuropilin and tolloid-like protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NETO1 recombinant protein (Position: R5-R514) was used as the immunogen for the NETO1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NETO1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NETO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NETO1

Target: NETO1 (Neuropilin (NRP) and Tolloid (TLL)-Like 1 (NETO1))

Andere Bezeichnung: NETO1

Hintergrund: NETO1 antibody detects Neuropilin and tolloid-like protein 1, a neuronal transmembrane glycoprotein that modulates ionotropic glutamate receptor function and synaptic plasticity. NETO1 acts as an auxiliary subunit of kainate-type glutamate receptors (KARs), regulating receptor trafficking, channel gating, and synaptic localization. The NETO1 antibody is widely used in neurobiology and synaptic physiology to study excitatory neurotransmission and learning mechanisms.

NETO1 is encoded by the NETO1 gene located on human chromosome 18q22.1. The protein is approximately 533 amino acids long and contains two extracellular CUB domains, a low-density lipoprotein receptor-like domain, a single transmembrane helix, and a short cytoplasmic tail. NETO1 is predominantly expressed in the hippocampus, cortex, and cerebellum, where it localizes to postsynaptic membranes and interacts with KAR subunits such as GluK2 and GluK5.

The NETO1 antibody detects a 63 kilodalton protein by western blot and shows punctate synaptic staining in neurons. NETO1 enhances the stability and functional diversity of KARs by modulating desensitization kinetics and surface expression. It contributes to long-term potentiation and synaptic scaling, processes underlying memory and learning. Mice lacking NETO1 display deficits in synaptic plasticity and spatial learning, confirming its critical role in excitatory neurotransmission.

Beyond the brain, NETO1 may participate in developmental signaling and axonal growth. Dysregulation or mutation of NETO1 has been associated with autism spectrum disorder, schizophrenia, and cognitive impairment. By shaping receptor signaling and synaptic strength, NETO1 maintains proper excitatory balance within neural circuits.

As a neuron-specific regulatory subunit of kainate receptors, NETO1 is a key molecule for studying excitatory synaptic physiology and plasticity. NSJ Bioreagents provides a validated NETO1 antibody optimized for its applications, supporting research into neurotransmission, synaptic regulation, and neurological disorders.

UniProt: Q8TDF5

Pathways: Regulation of long-term Neuronal Synaptic Plasticity