Polybromo 1 Antikörper (AA 45-1647)

Produktnummer ABIN7875057

Der Kaninchen Polyklonal anti-Polybromo 1 Antikörper wird verwendet zum Nachweis von Polybromo 1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, FACS und ELISA.

Kurzübersicht für Polybromo 1 Antikörper (AA 45-1647) (ABIN7875057)

Target: Polybromo 1 (PBRM1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Polybromo 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Produktdetails anti-Polybromo 1 Antikörper

Bindungsspezifität: AA 45-1647

Verwendungszweck: PBRM1 Antibody / Polybromo 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human PBRM1 recombinant protein (Position: D45-R1647) was used as the immunogen for the PBRM1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PBRM1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PBRM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu Polybromo 1

Target: Polybromo 1 (PBRM1)

Andere Bezeichnung: PBRM1

Hintergrund: PBRM1 antibody detects Polybromo-1, a chromatin-remodeling factor and tumor suppressor that regulates gene expression through nucleosome repositioning. The UniProt recommended name is Polybromo-1 (PBRM1). This large nuclear protein functions as a subunit of the PBAF (polybromo-associated BRG1-associated factor) subtype of the SWI/SNF chromatin-remodeling complex, which modulates chromatin accessibility to control transcription, differentiation, and DNA repair.

Functionally, PBRM1 antibody identifies a 1,709-amino-acid protein containing six bromodomains, two BAH domains, and one HMG-box domain that recognize acetylated lysine residues on histones. These interactions recruit the PBAF complex to specific promoters, enabling ATP-dependent chromatin remodeling and transcriptional regulation. PBRM1 supports cellular homeostasis by maintaining chromatin architecture and transcriptional fidelity.

The PBRM1 gene is located on chromosome 3p21.1 and is highly expressed in kidney, liver, and lung tissues. It acts as a critical tumor suppressor by opposing oncogenic transcriptional programs, particularly in renal and hepatic cells.

Pathologically, PBRM1 mutations are frequently observed in clear cell renal cell carcinoma (ccRCC), intrahepatic cholangiocarcinoma, and other malignancies. Loss of PBRM1 disrupts chromatin remodeling, leading to transcriptional dysregulation and uncontrolled cell proliferation. Research using PBRM1 antibody supports studies in epigenetics, tumor suppression, and chromatin dynamics.

PBRM1 antibody is validated for western blotting, immunoprecipitation, and immunohistochemistry to detect chromatin-remodeling complex components. NSJ Bioreagents provides PBRM1 antibody reagents optimized for transcriptional regulation, chromatin accessibility, and cancer epigenomics research.

Structurally, Polybromo-1 features multiple bromodomains that bind acetylated histones and a modular organization that promotes assembly with PBAF complex members such as BRD7 and SMARCA4. This antibody facilitates exploration of PBRM1's role in chromatin organization and gene silencing.

UniProt: Q86U86