LMO2 Antikörper (AA 4-150)

Produktnummer ABIN7874657

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LMO2 in WB, FACS, ELISA und IP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für LMO2 Antikörper (AA 4-150) (ABIN7874657)

Target: LMO2 (LIM Domain Only 2 (Rhombotin-Like 1) (LMO2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LMO2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP)

Produktdetails anti-LMO2 Antikörper

Bindungsspezifität: AA 4-150

Verwendungszweck: LMO2 Antibody / Rhombotin 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human LMO2 recombinant protein (Position: A4-E150) was used as the immunogen for the LMO2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LMO2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the LMO2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu LMO2

Target: LMO2 (LIM Domain Only 2 (Rhombotin-Like 1) (LMO2))

Andere Bezeichnung: LMO2

Hintergrund: LMO2 antibody detects LIM domain only protein 2, encoded by the LMO2 gene on chromosome 11p13. LMO2 antibody is commonly applied in research on hematopoiesis, transcriptional regulation, and cancer biology. LMO2 is a transcriptional co-regulator without intrinsic DNA-binding activity, instead, it functions as an adaptor protein that brings together DNA-binding transcription factors within multiprotein complexes. It plays essential roles in hematopoietic stem cell maintenance, vascular development, and T-cell differentiation. Expression is high in hematopoietic progenitors and endothelial cells, but tightly regulated, as dysregulation promotes oncogenesis.

Structurally, LMO2 is a small nuclear protein of ~158 amino acids containing two tandem LIM domains, zinc-binding motifs that mediate protein-protein interactions. These LIM domains allow LMO2 to interact with basic helix-loop-helix (bHLH) proteins such as TAL1, GATA1, and LDB1, forming complexes that regulate transcription of genes required for hematopoietic development. Alternative splicing generates isoforms with distinct interaction patterns.

Functionally, LMO2 acts as a bridging factor, linking transcription factors to DNA regulatory regions. It is essential for erythropoiesis and angiogenesis, controlling expression of genes involved in cell fate decisions. Knockout mice lacking LMO2 exhibit embryonic lethality due to failure of blood vessel and hematopoietic development. In hematopoietic stem cells, LMO2 ensures long-term maintenance and self-renewal. Researchers use LMO2 antibody to study transcriptional regulation in blood formation and cancer biology.

Clinically, LMO2 is a well-known oncogene in T-cell acute lymphoblastic leukemia (T-ALL), where aberrant expression drives leukemogenesis. Chromosomal translocations or retroviral insertional mutagenesis can activate LMO2, promoting proliferation of immature T-cells. It also has roles in diffuse large B-cell lymphoma and other hematologic malignancies. Conversely, in normal physiology, its expression serves as a marker for angiogenesis and hematopoietic stem cell activity. NSJ Bioreagents provides LMO2 antibody to support oncology and stem cell research.

Experimentally, LMO2 antibody is used in western blotting to detect the ~18 kDa protein, in immunohistochemistry to examine expression in hematopoietic and endothelial tissues, and in immunofluorescence microscopy to confirm nuclear localization. Co-immunoprecipitation with LMO2 antibody allows identification of transcriptional complexes and regulatory partners.

UniProt: P25791

Pathways: Chromatin Binding