WHSC2 Antikörper (AA 38-482)

Produktnummer ABIN7874510

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch WHSC2 in WB, ELISA und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für WHSC2 Antikörper (AA 38-482) (ABIN7874510)

Target: WHSC2 (Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WHSC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-WHSC2 Antikörper

Bindungsspezifität: AA 38-482

Verwendungszweck: NELFA Antibody / Negative elongation factor A

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NELFA recombinant protein (Position: I38-L482) was used as the immunogen for the NELFA antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NELFA antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NELFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu WHSC2

Target: WHSC2 (Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2))

Andere Bezeichnung: NELFA

Hintergrund: NELFA antibody detects Negative elongation factor A, a transcriptional regulator that controls RNA polymerase II pausing and transcription elongation. Encoded by the NELFA gene on chromosome 9q34.11, this protein is a core component of the negative elongation factor (NELF) complex, which also includes NELFB, NELFC/D, and NELFE subunits. Together, these proteins transiently inhibit RNA polymerase II progression downstream of transcription start sites, enabling proper promoter-proximal pausing, RNA capping, and regulated gene expression.

NELFA interacts with DSIF (DRB sensitivity-inducing factor) and phosphorylated RNA polymerase II to modulate transcriptional timing and elongation efficiency. Release from pausing is mediated by P-TEFb-dependent phosphorylation events that allow productive elongation. Through these mechanisms, NELFA coordinates transcriptional responses to developmental, environmental, and signaling cues.

The NELFA antibody is widely used in transcription, chromatin, and epigenetic regulation research to study RNA polymerase II pausing, elongation control, and transcriptional activation. Western blot analysis identifies a 66 kilodalton band corresponding to NELFA, while immunofluorescence reveals nuclear punctate staining associated with active transcription foci. This antibody supports studies investigating transcriptional regulation and pause-release mechanisms.

Altered NELFA activity affects global gene expression programs, contributing to developmental disorders, cancer, and viral replication control. NELFA has also been linked to stress responses, where it modulates transcription restart following DNA damage or heat shock. The NELFA antibody provides a critical reagent for dissecting the molecular mechanisms of transcription elongation and RNA polymerase II regulation. NSJ Bioreagents offers this antibody validated for its applications, ensuring dependable performance in transcriptional research.

UniProt: Q9H3P2

Pathways: Chromatin Binding