AGO1 Antikörper (AA 377-410)

Produktnummer ABIN7874474

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch AGO1 in WB. Es zeigt Reaktivität gegenüber Proben von Zebrafisch (Danio rerio).

Kurzübersicht für AGO1 Antikörper (AA 377-410) (ABIN7874474)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reaktivität: Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-AGO1 Antikörper

Bindungsspezifität: AA 377-410

Verwendungszweck: Zebrafish Argonaute 1 Antibody / Ago1

Aufreinigung: Antigen affinity chromatography

Immunogen: An E.coli-derived zebrafish Argonaute 1 recombinant protein (amino acids E377-R410) was used as the immunogen for the Zebrafish Argonaute 1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Zebrafish Argonaute 1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the Zebrafish Argonaute 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Andere Bezeichnung: Argonaute 1

Hintergrund: Argonaute 1 (Ago1) is a core component of the RNA-induced silencing complex (RISC), playing a pivotal role in post-transcriptional gene regulation through small RNA pathways. In zebrafish, Ago1 is integral to microRNA (miRNA)-mediated gene silencing and participates in processes crucial for embryonic development, cellular differentiation, and gene expression regulation.

Biological Function:
Ago1 binds to small RNAs (miRNAs and siRNAs) and guides RISC to target messenger RNAs (mRNAs) for translational repression or degradation. In zebrafish, Ago1 is particularly important for miRNA function during early development, where it helps regulate maternal mRNA clearance and zygotic gene activation. Functional studies have shown that loss of Ago1 impairs embryonic patterning, cell movement, and germ layer formation.

Structure and Domains:
Zebrafish Ago1 contains the characteristic PAZ and PIWI domains. The PAZ domain anchors the 3' end of small RNAs, while the PIWI domain has RNase H-like activity, enabling mRNA cleavage in certain contexts.

Expression Pattern:
Ago1 mRNA is maternally deposited and broadly expressed during early embryogenesis. Later, its expression is enriched in proliferative and neural tissues, reflecting its role in controlling gene expression during development and differentiation.

Orthology and Conservation:
Zebrafish Ago1 is highly conserved with its mammalian counterparts, including human AGO1. This conservation supports the use of zebrafish as a model for studying RNA interference (RNAi), small RNA pathways, and related genetic regulatory mechanisms.

UniProt: K4I6K9

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization