MCM4 Antikörper (AA 361-677)

Produktnummer ABIN7874331

Der Kaninchen Polyklonal anti-MCM4 Antikörper wird verwendet zum Nachweis von MCM4 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF, IHC (p) und FACS.

Kurzübersicht für MCM4 Antikörper (AA 361-677) (ABIN7874331)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Produktdetails anti-MCM4 Antikörper

Bindungsspezifität: AA 361-677

Verwendungszweck: MCM4 Antibody

Aufreinigung: Antigen affinity

Immunogen: Recombinant human partial protein (amino acids 361-677) was used as the immunogen for this MCM4 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the MCM4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MCM4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Andere Bezeichnung: MCM4

Hintergrund: Minichromosome maintenance, S. cerevisiae, homolog of, 4, also called CDC21, is a protein that in humans is encoded by the MCM4 gene. MCM4 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The 864-amino acid protein has an observed molecular mass of 97 kD by SDS-PAGE, which is similar to its calculated molecular mass of 96.6 kD. Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells. In the absence of DDK, CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial. The gene encodes a subunit of the MCM2-7 complex (also known as MCM2-MCM7), the replication licensing factor and presumptive replicative helicase.

UniProt: P33991

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA