DDX39B Antikörper (AA 351-380)

Produktnummer ABIN7874225

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DDX39B in WB, FACS und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für DDX39B Antikörper (AA 351-380) (ABIN7874225)

Target: DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDX39B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DDX39B Antikörper

Bindungsspezifität: AA 351-380

Verwendungszweck: DDX39B Antibody / UAP56

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 351-380 from the human protein was used as the immunogen for the DDX39B antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the DDX39B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the DDX39B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Details zu DDX39B

Target: DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))

Andere Bezeichnung: DDX39B

Hintergrund: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing-and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. It has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. It can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. It can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.

UniProt: Q13838

Pathways: Ribonucleoprotein Complex Subunit Organization