UBR5 Antikörper (AA 31-761)

Produktnummer ABIN7873803

Der Kaninchen Polyklonal anti-UBR5 Antikörper wird verwendet zum Nachweis von UBR5 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für UBR5 Antikörper (AA 31-761) (ABIN7873803)

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBR5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-UBR5 Antikörper

Bindungsspezifität: AA 31-761

Verwendungszweck: UBR5 Antibody / hHYD

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human UBR5 recombinant protein (Position: K31-K761) was used as the immunogen for the UBR5 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the UBR5 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the UBR5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu UBR5

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Andere Bezeichnung: UBR5

Hintergrund: UBR5 antibody detects E3 ubiquitin-protein ligase UBR5, a large multidomain enzyme that catalyzes ubiquitination of specific substrate proteins, regulating DNA damage repair, transcription, and cell cycle control. UBR5 belongs to the HECT-type E3 ligase family and plays a critical role in maintaining genomic integrity and proteostasis. The UBR5 antibody is widely used in studies of ubiquitin signaling, cancer biology, and DNA repair mechanisms.

UBR5 is encoded by the UBR5 gene located on human chromosome 8q22.3. The protein is approximately 300 kilodaltons and contains a C-terminal HECT domain responsible for ubiquitin transfer, an N-terminal UBR box involved in substrate recognition, and several WW domains that mediate protein-protein interactions. UBR5 localizes to both the nucleus and cytoplasm, functioning in proteasomal degradation and signaling complex assembly.

The UBR5 antibody detects a 300 kilodalton band in western blot analysis and shows both nuclear and cytoplasmic staining under immunofluorescence. UBR5 ubiquitinates regulators of the DNA damage response such as BRCA1 and p53, coordinating checkpoint activation and repair. It also targets transcription factors and signaling intermediates, integrating stress responses with transcriptional control.

UBR5 is essential for embryonic development and cell viability. Its dysregulation is associated with multiple cancers, including breast, ovarian, and colorectal carcinoma, where it functions as either an oncogene or tumor suppressor depending on context. Overexpression enhances proliferation and metastasis, while depletion sensitizes cells to DNA-damaging agents and promotes apoptosis.

As a master regulator of ubiquitin signaling, UBR5 links proteolytic control with cell fate decisions. NSJ Bioreagents provides a validated UBR5 antibody optimized for western blot and flow cytometry, supporting research into ubiquitination pathways, DNA damage responses, and oncogenic signaling networks.

UniProt: O95071

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway