FAM59A Antikörper (AA 277-753)

Produktnummer ABIN7873213

Der Kaninchen Polyklonal anti-FAM59A Antikörper (ABIN7873213) detektiert spezifisch FAM59A in ELISA, WB, IHC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für FAM59A Antikörper (AA 277-753) (ABIN7873213)

Target: FAM59A (Family with Sequence Similarity 59, Member A (FAM59A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FAM59A Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-FAM59A Antikörper

Bindungsspezifität: AA 277-753

Verwendungszweck: GAREM1 Antibody / GRB2-associated and regulator of MAPK protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human GAREM1 recombinant protein (Position: Q277-E753) was used as the immunogen for the GAREM1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the GAREM1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the GAREM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu FAM59A

Target: FAM59A (Family with Sequence Similarity 59, Member A (FAM59A))

Andere Bezeichnung: GAREM1

Hintergrund: GAREM1 antibody detects GRB2-associated regulator of MAPK1 subtype 1, an adaptor protein involved in growth factor receptor signaling, particularly within the epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor (FGFR) pathways. GAREM1 acts as a downstream effector of receptor tyrosine kinases and plays a role in mitogen-activated protein kinase (MAPK) signaling, cellular proliferation, and differentiation. The GAREM1 antibody is used to investigate mechanisms of growth factor signaling, cell migration, and oncogenic transformation.

GAREM1 is encoded by the GAREM1 gene located on human chromosome 18p11.32. The protein is approximately 95 kilodaltons and contains multiple functional regions, including a pleckstrin homology (PH) domain, proline-rich motifs, and SH3-binding sequences that facilitate interaction with growth factor receptor-bound protein 2 (GRB2). Through these interactions, GAREM1 acts as a scaffolding adaptor that transmits extracellular growth factor signals to downstream MAPK/ERK cascades, modulating transcriptional responses and cytoskeletal reorganization.

The GAREM1 antibody detects a 90-100 kilodalton protein on western blot and reveals cytoplasmic and perinuclear localization by immunofluorescence. Activation of EGFR or FGFR promotes tyrosine phosphorylation of GAREM1, which enhances its association with signaling molecules such as SHP2, ERK1/2, and SOS1. Functionally, GAREM1 contributes to cell cycle progression and survival, and its overexpression has been observed in certain cancers, including hepatocellular carcinoma and glioblastoma. Depletion or inhibition of GAREM1 disrupts ERK activation, leading to reduced cell proliferation and migration.

GAREM1 also influences differentiation processes by regulating cytoskeletal dynamics and cell adhesion. In neurons, GAREM1 modulates neurite outgrowth and receptor clustering, linking receptor tyrosine kinase signaling to actin remodeling. It may further act as a regulatory hub integrating MAPK and PI3K signaling pathways. NSJ Bioreagents provides a validated GAREM1 antibody optimized for western blot, immunohistochemistry, and immunofluorescence, supporting studies of receptor signaling, cancer cell proliferation, and cellular differentiation.

UniProt: Q9H706

Pathways: EGFR Signaling Pathway