RAD51 Homolog B Antikörper (AA 22-384)

Produktnummer ABIN7872323

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RAD51 Homolog B in WB, ELISA, IF, FACS und ICC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für RAD51 Homolog B Antikörper (AA 22-384) (ABIN7872323)

Target: RAD51 Homolog B (Rad51B)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD51 Homolog B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-RAD51 Homolog B Antikörper

Bindungsspezifität: AA 22-384

Verwendungszweck: RAD51B Antibody / RAD51L1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RAD51L1/RAD51B recombinant protein (Position: H22-F384) was used as the immunogen for the RAD51B antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RAD51B antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RAD51B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RAD51 Homolog B

Target: RAD51 Homolog B (Rad51B)

Andere Bezeichnung: RAD51B

Hintergrund: RAD51B antibody detects DNA repair protein RAD51 homolog 2, encoded by the RAD51B gene (also known as RAD51L1) on chromosome 14q24.1. RAD51B antibody is widely used in DNA damage response, homologous recombination, and cancer biology research. RAD51B is part of the RAD51 paralog family, which functions in homologous recombination repair of DNA double-strand breaks. It acts as a mediator, promoting the assembly and stability of RAD51 nucleoprotein filaments on single-stranded DNA.

Structurally, RAD51B is a ~42 kDa protein with ATP-binding motifs and DNA-binding domains. It forms a heterodimer with RAD51C and participates in the BCDX2 complex with RAD51C, RAD51D, and XRCC2, as well as in the CX3 complex with RAD51C and XRCC3. These complexes support RAD51 loading at DNA damage sites and stabilize recombination intermediates.

Functionally, RAD51B contributes to DNA repair fidelity by ensuring accurate homologous recombination. Loss of RAD51B impairs recombination, leading to chromosomal instability, hypersensitivity to DNA-damaging agents, and defective cell cycle checkpoints. Researchers use RAD51B antibody to study DNA repair, recombination, and genome stability.

Clinically, RAD51B variants are associated with breast cancer susceptibility and other malignancies. Disruption of RAD51B function contributes to genomic instability, a hallmark of cancer. Because homologous recombination is a therapeutic target in oncology, RAD51B is of interest in studies of PARP inhibitor sensitivity and synthetic lethality. NSJ Bioreagents provides RAD51B antibody for DNA repair and cancer research.

Experimentally, RAD51B antibody is applied in western blotting to detect the ~42 kDa protein, in immunofluorescence to study DNA repair foci, and in co-immunoprecipitation to analyze protein complexes. Chromatin immunoprecipitation with RAD51B antibody supports studies of recombination site localization.

UniProt: O15315

Pathways: DNA Reparatur