SETDB1 Antikörper (AA 20-871)

Produktnummer ABIN7871887

Der Kaninchen Polyklonal anti-SETDB1 Antikörper (ABIN7871887) detektiert spezifisch SETDB1 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für SETDB1 Antikörper (AA 20-871) (ABIN7871887)

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SETDB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-SETDB1 Antikörper

Bindungsspezifität: AA 20-871

Verwendungszweck: SETDB1 Antibody / Histone-lysine N-methyltransferase SETDB1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SETDB1 recombinant protein (Position: E20-E871) was used as the immunogen for the SETDB1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SETDB1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SETDB1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SETDB1

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Andere Bezeichnung: SETDB1

Hintergrund: SETDB1 antibody detects Histone-lysine N-methyltransferase SETDB1, an enzyme responsible for methylating histone H3 at lysine 9 (H3K9), a key epigenetic mark associated with transcriptional repression and heterochromatin formation. Encoded by the SETDB1 gene on chromosome 1q21.3, this nuclear protein is part of the SET domain-containing family of histone methyltransferases and plays a central role in chromatin remodeling, gene silencing, and genome stability.

SETDB1 contains a SET domain conferring methyltransferase activity, a methyl-CpG-binding domain (MBD), and a tudor domain that facilitate chromatin targeting. It forms complexes with co-repressors such as KAP1 (TRIM28) and HP1, directing H3K9 trimethylation at retroelements, developmental genes, and repetitive sequences. Through this mechanism, SETDB1 maintains transcriptional repression and contributes to X-chromosome inactivation, embryonic stem cell identity, and defense against endogenous retroviral elements.

The SETDB1 antibody is widely used in epigenetics, developmental biology, and cancer research to examine histone methylation and transcriptional silencing. Western blot analysis identifies a 170 kilodalton band corresponding to SETDB1, while immunofluorescence reveals punctate nuclear staining colocalizing with heterochromatin regions. This antibody enables researchers to assess chromatin states, epigenetic modifications, and silencing pathways across diverse cell types.

Aberrant SETDB1 expression and activity have been linked to multiple cancers, including melanoma, glioma, and lung carcinoma, where it promotes oncogenic transcriptional reprogramming and genomic instability. SETDB1 overexpression can also suppress immune responses by silencing interferon-stimulated genes. The SETDB1 antibody provides a precise reagent for studying epigenetic repression, gene regulation, and chromatin-based disease mechanisms. NSJ Bioreagents validates this antibody for its applications, ensuring high specificity for epigenetic and transcriptional research.

UniProt: Q15047