SYCN Antikörper (AA 19-134)

Produktnummer ABIN7871559

Der Kaninchen Polyklonal anti-SYCN Antikörper wird verwendet zum Nachweis von SYCN in Proben von Human, Maus und Ratte. Er wurde validiert für ELISA, WB, ICC, FACS und IF.

Kurzübersicht für SYCN Antikörper (AA 19-134) (ABIN7871559)

Target: SYCN (Syncollin (SYCN))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SYCN Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-SYCN Antikörper

Bindungsspezifität: AA 19-134

Verwendungszweck: SYCN Antibody / Syncollin

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SYCN recombinant protein (Position: A19-S134) was used as the immunogen for the SYCN antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SYCN antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SYCN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SYCN

Target: SYCN (Syncollin (SYCN))

Andere Bezeichnung: SYCN

Hintergrund: SYCN antibody detects Syncollin, a secretory vesicle membrane protein that plays an important role in pancreatic enzyme secretion and zymogen granule exocytosis. Encoded by the SYCN gene on chromosome 19q13.33, Syncollin is a small protein localized to the membranes of secretory vesicles within pancreatic acinar cells and other exocrine tissues. It functions as an accessory factor that promotes granule maturation, docking, and fusion with the plasma membrane during regulated secretion.

Structurally, Syncollin contains a coiled-coil domain responsible for oligomerization and membrane tethering, allowing it to form homo-oligomeric complexes on the luminal surface of zymogen granules. These complexes interact with SNARE proteins and other components of the exocytic machinery to facilitate vesicle fusion and enzyme release. SYCN contributes to the organization of zymogen granule membranes and ensures precise regulation of digestive enzyme secretion in response to cholecystokinin and other secretagogues.

The SYCN antibody is widely used in cell biology, gastrointestinal physiology, and secretion research to investigate vesicle dynamics, exocytosis, and pancreatic function. Western blot analysis detects a 13 kilodalton band corresponding to Syncollin, while immunofluorescence reveals punctate staining in the apical cytoplasm of acinar cells and along the zymogen granule membranes. This antibody supports analysis of regulated secretory pathways and granule maturation in exocrine tissues.

Disruption or reduced expression of SYCN impairs vesicle exocytosis and digestive enzyme secretion, leading to altered pancreatic physiology. In experimental models, loss of Syncollin function results in defective granule docking and diminished amylase release, highlighting its essential role in the secretory machinery. The SYCN antibody provides a robust reagent for examining vesicle organization, exocytotic mechanisms, and the structural biology of secretory organelles. NSJ Bioreagents validates this antibody for its applications to ensure reliable and reproducible results for studies on secretion and exocrine function.

UniProt: Q0VAF6