CDKN2AIP Antikörper (AA 183-689)

Produktnummer ABIN7871471

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CDKN2AIP in WB, ELISA und FACS. Es zeigt Reaktivität gegenüber Proben von Maus und Ratte.

Kurzübersicht für CDKN2AIP Antikörper (AA 183-689) (ABIN7871471)

Target: CDKN2AIP (CDKN2A Interacting Protein (CDKN2AIP))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDKN2AIP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-CDKN2AIP Antikörper

Bindungsspezifität: AA 183-689

Verwendungszweck: Carf Antibody / Collaborator of ARF / Cdkn2aip

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived mouse CDKN2AIP/CARF recombinant protein (Position: R183-T689) was used as the immunogen for the Carf antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Carf antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the Carf antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu CDKN2AIP

Target: CDKN2AIP (CDKN2A Interacting Protein (CDKN2AIP))

Andere Bezeichnung: Carf

Hintergrund: Carf antibody detects Collaborator of ARF, a nuclear protein encoded by the CDKN2AIP gene on chromosome 4q35.1. CARF (CDKN2A-interacting protein) acts as a transcriptional co-regulator and cell cycle checkpoint factor that mediates the tumor suppressor function of p14ARF and p53. It plays key roles in cellular senescence, DNA damage response, and cell proliferation control. CARF is expressed in most proliferative tissues, including liver, kidney, and hematopoietic cells, with particularly high levels in dividing and stress-activated cells.

Structurally, CARF contains a coiled-coil domain and nuclear localization signals that facilitate interaction with chromatin-associated proteins and transcriptional regulators. It belongs to the CDKN2A-interacting protein family and acts as a bridge between the ARF tumor suppressor pathway and p53 signaling. CARF directly binds p14ARF and modulates p53 stability through MDM2 regulation, coordinating cell cycle arrest in response to genotoxic stress. Co-localization studies show CARF within the nucleus and nucleolus, where it associates with replication machinery and chromatin-modifying complexes.

Functionally, CARF serves as a cell cycle regulator that maintains p53 activity and prevents uncontrolled proliferation. Under DNA damage conditions, CARF enhances p53-dependent transcription of target genes such as CDKN1A (p21), promoting cell cycle arrest and senescence. It also regulates replication stress responses by interacting with checkpoint proteins such as ATR and CHK1. CARF expression levels determine whether a cell undergoes reversible arrest or permanent senescence, integrating multiple stress and growth signals.

Dysregulation of CARF expression has been implicated in cancer and aging. Overexpression can suppress proliferation and induce senescence, while loss of CARF may impair p53 function and enhance oncogenic transformation. In certain tumors, CARF mislocalization correlates with altered cell cycle control. Pathway associations include p53 signaling, DNA damage response, and cell cycle checkpoint regulation. Developmentally, CARF contributes to tissue homeostasis by regulating stem and progenitor cell proliferation.

The Carf antibody from NSJ Bioreagents is a valuable reagent for studies of tumor suppression, senescence, and p53 pathway regulation.

UniProt: Q8VHI4