Melanoma gp100 Antikörper (AA 182-5565)

Produktnummer ABIN7871468

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Melanoma gp100 in IHC, WB, FACS, IF und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für Melanoma gp100 Antikörper (AA 182-5565) (ABIN7871468)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Melanoma gp100 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Produktdetails anti-Melanoma gp100 Antikörper

Bindungsspezifität: AA 182-5565

Verwendungszweck: PMEL Antibody / Premelanosome protein / SILV

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SILV/PMEL recombinant protein (Position: H182-Q5565) was used as the immunogen for the PMEL antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PMEL antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PMEL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Andere Bezeichnung: PMEL

Hintergrund: PMEL antibody detects Premelanosome protein, a structural component of melanosomes essential for melanin synthesis and pigment formation. The UniProt recommended name is Premelanosome protein (PMEL). Also known as SILV, gp100, or ME20, this glycoprotein forms amyloid fibrils that serve as scaffolds for melanin polymerization within developing melanosomes.

Functionally, PMEL antibody identifies a 661-amino-acid type I transmembrane glycoprotein synthesized in melanocytes. PMEL is cleaved by proprotein convertases into M alpha and M beta fragments, which assemble into amyloid fibrils in stage II melanosomes. These fibrils provide a matrix for eumelanin deposition, enabling proper pigment granule maturation.

The PMEL gene is located on chromosome 12p13.31 and is expressed exclusively in pigment-producing cells, including melanocytes and retinal pigment epithelium. PMEL expression is regulated by the transcription factor MITF and is upregulated during melanocyte differentiation. It plays a central role in pigment biogenesis and melanosome architecture.

Pathologically, PMEL is a target antigen in melanoma immunotherapy and an established marker for melanocytic tumors. Mutations in PMEL can cause pigment dilution phenotypes in animals due to altered fibril formation. Research using PMEL antibody supports studies in pigmentation biology, melanoma diagnostics, and organelle structure.

PMEL antibody is validated for immunohistochemistry, western blotting, and immunofluorescence to detect melanosomal structural proteins. NSJ Bioreagents provides PMEL antibody reagents optimized for pigment biology, cancer research, and cell differentiation studies.

Structurally, Premelanosome protein contains an N-terminal signal peptide, a polycystic kidney disease-like (PKD) domain, and a transmembrane domain. Its cleaved fragments aggregate to form functional amyloid fibrils. This antibody supports detailed examination of PMEL's role in melanosome assembly and pigmentation control.

UniProt: P40967