PFDN2 Antikörper (AA 18-98)

Produktnummer ABIN7871436

Der Kaninchen Polyklonal anti-PFDN2 Antikörper wird verwendet zum Nachweis von PFDN2 in Proben von Human und Ratte. Er wurde validiert für WB, ELISA, IF, ICC und FACS.

Kurzübersicht für PFDN2 Antikörper (AA 18-98) (ABIN7871436)

Target: PFDN2 (Prefoldin Subunit 2 (PFDN2))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PFDN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-PFDN2 Antikörper

Bindungsspezifität: AA 18-98

Verwendungszweck: PFDN2 Antibody / Prefoldin subunit 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human PFDN2 recombinant protein (Position: K18-Q98) was used as the immunogen for the PFDN2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PFDN2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PFDN2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PFDN2

Target: PFDN2 (Prefoldin Subunit 2 (PFDN2))

Andere Bezeichnung: PFDN2

Hintergrund: PFDN2 antibody detects Prefoldin subunit 2, a cytoplasmic molecular chaperone encoded by the PFDN2 gene on chromosome 1q23.3. PFDN2 is an essential component of the prefoldin complex, a hexameric cochaperone that assists in folding nascent polypeptides and preventing aggregation of cytoskeletal proteins such as actin and tubulin. The prefoldin complex captures newly synthesized, unfolded proteins and transfers them to the group II chaperonin CCT (TCP-1 ring complex) for final folding, ensuring proper assembly of cytoskeletal and structural proteins required for cell function.

By stabilizing actin and tubulin subunits, PFDN2 supports cytoskeletal organization, cell division, and intracellular transport. Its role as a chaperone subunit is central to maintaining proteostasis, allowing cells to manage misfolded proteins during stress conditions. PFDN2 expression is ubiquitous and tightly regulated to sustain cytoskeletal dynamics across tissues, particularly in neurons, epithelial cells, and fibroblasts.

Structurally, PFDN2 forms one arm of the jellyfish-shaped prefoldin complex, which consists of six subunits (PFDN1-6) arranged in a heterohexameric structure. This configuration provides flexibility for binding diverse substrates. PFDN2 contains coiled-coil and beta-hairpin motifs that mediate interactions with unfolded protein substrates, helping maintain their solubility until delivery to the CCT complex. Its conservation across eukaryotes highlights its fundamental role in protein quality control and cellular stability.

PFDN2 also participates in transcriptional regulation and cellular stress responses, possibly through nuclear interactions under certain conditions. It functions within the broader protein folding and proteostasis network, cooperating with heat shock proteins such as HSP70 and chaperonins to maintain a balanced proteome. Disruption of PFDN2 or other prefoldin subunits leads to cytoskeletal instability, reduced cell proliferation, and heightened sensitivity to proteotoxic stress.

Dysregulation of PFDN2 expression has been linked to several diseases. Reduced prefoldin activity can contribute to neurodegenerative disorders where protein misfolding is pathogenic, such as Parkinson's and Huntington's disease. Overexpression of PFDN2 has been noted in certain cancers, including hepatocellular and colorectal carcinoma, where enhanced folding capacity may support tumor cell growth and resistance to stress. PFDN2 operates in the protein folding and cytoskeletal assembly pathways, underscoring its role in maintaining cell shape and function.

Immunohistochemical staining using PFDN2 antibody demonstrates cytoplasmic localization consistent with its chaperone activity. The PFDN2 antibody from NSJ Bioreagents is a valuable reagent for studying protein folding mechanisms, cytoskeletal organization, and proteostasis in normal and disease conditions.

UniProt: Q9UHV9

Pathways: Unfolded Protein Response