MATK Antikörper (AA 18-298)

Produktnummer ABIN7871404

Der Kaninchen Polyklonal anti-MATK Antikörper (ABIN7871404) detektiert spezifisch MATK in WB, IHC, ELISA, ICC, FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für MATK Antikörper (AA 18-298) (ABIN7871404)

Target: MATK (Megakaryocyte-Associated tyrosine Kinase (MATK))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MATK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-MATK Antikörper

Bindungsspezifität: AA 18-298

Verwendungszweck: MATK Antibody / Megakaryocyte-associated tyrosine kinase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human MATK recombinant protein (Position: S18-Q298) was used as the immunogen for the MATK antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MATK antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MATK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MATK

Target: MATK (Megakaryocyte-Associated tyrosine Kinase (MATK))

Andere Bezeichnung: MATK

Hintergrund: MATK antibody detects Megakaryocyte-associated tyrosine kinase, a cytoplasmic kinase encoded by the MATK gene located on chromosome 19q13.3. MATK belongs to the Csk (C-terminal Src kinase) family of non-receptor tyrosine kinases, acting primarily as a negative regulator of Src family kinases such as LYN, FYN, and HCK. It is expressed in hematopoietic cells, especially platelets, T cells, B cells, and megakaryocytes, where it modulates signaling cascades that control cell activation, proliferation, and differentiation. MATK serves as a key regulator of immune cell signaling and platelet function by maintaining balanced tyrosine phosphorylation levels.

Structurally, MATK contains an SH3 domain, an SH2 domain, and a C-terminal catalytic kinase domain, similar to Csk, but it lacks the N-terminal myristoylation signal that targets Csk to membranes. This structural difference restricts MATK mainly to the cytoplasm, where it regulates Src kinases indirectly through adaptor-mediated interactions. MATK belongs to the protein tyrosine kinase family and shares homology with other signaling regulators such as CSK and CHK. Known interacting partners include PAG1, LAT, and LYN, which facilitate recruitment to signaling complexes.

Functionally, MATK phosphorylates inhibitory tyrosine residues within Src family kinases, leading to their inactivation and termination of receptor signaling. In platelets, MATK regulates activation by suppressing FYN and LYN kinase signaling downstream of GPVI and integrins. In immune cells, it modulates T-cell receptor (TCR) and B-cell receptor (BCR) signaling, helping to prevent overactivation and maintain immune tolerance. MATK also participates in neuronal and endothelial cell signaling, contributing to cytoskeletal organization and vascular homeostasis.

MATK plays an important role in preventing hyperactivation of immune responses and maintaining hemostatic balance. Dysregulation of MATK expression or activity has been associated with autoimmune disorders, inflammatory diseases, and malignancies. Reduced MATK expression can result in excessive Src kinase activity, contributing to aberrant cell growth and survival. Conversely, overexpression has been observed in certain leukemias, where it suppresses proliferation signaling. Pathway involvement includes Src kinase signaling, platelet activation, and immune receptor regulation.

The MATK antibody from NSJ Bioreagents is an excellent reagent for studying tyrosine kinase signaling, immune regulation, and platelet biology.

UniProt: P42679