BIM Antikörper (AA 18-186)

Produktnummer ABIN7871398

Der Kaninchen Polyklonal anti-BIM Antikörper (ABIN7871398) detektiert spezifisch BIM in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für BIM Antikörper (AA 18-186) (ABIN7871398)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BIM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-BIM Antikörper

Bindungsspezifität: AA 18-186

Verwendungszweck: Bim Antibody / Bcl-2-like protein 11

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived mouse Bim/Bcl2l11 recombinant protein (Position: Q18-R186) was used as the immunogen for the Bim antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Bim antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the Bim antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Andere Bezeichnung: Bim

Hintergrund: Bim antibody detects Bcl-2-like protein 11, a pro-apoptotic member of the Bcl-2 family that promotes programmed cell death through mitochondrial outer membrane permeabilization. The UniProt recommended name is Bcl-2-like protein 11 (BCL2L11). Also known as Bim, this BH3-only protein is a critical initiator of apoptosis in response to cellular stress, cytokine withdrawal, or DNA damage.

Functionally, Bim antibody identifies a 198-amino-acid cytoplasmic protein that binds anti-apoptotic Bcl-2 proteins (such as BCL2, BCL-XL, and MCL1), releasing pro-apoptotic effectors BAX and BAK to trigger mitochondrial cytochrome c release and caspase activation. Bim exists in several isoforms (BimEL, BimL, and BimS) generated by alternative splicing, which modulate apoptosis sensitivity across cell types.

The BCL2L11 gene is located on chromosome 2q13 and is expressed in lymphoid, neuronal, and epithelial tissues. Its expression is tightly regulated by survival pathways including PI3K/AKT and MAPK signaling, which control phosphorylation and proteasomal degradation of Bim.

Pathologically, dysregulation of Bim contributes to cancer, autoimmune diseases, and neurodegeneration. Reduced Bim expression confers resistance to apoptosis in tumor cells, while overexpression causes excessive neuronal death. Research using Bim antibody supports studies in cell death signaling, oncology, and stress-induced apoptosis.

Bim antibody is validated for western blotting, immunohistochemistry, and apoptosis assays to detect Bcl-2 family proteins. NSJ Bioreagents provides Bim antibody reagents optimized for apoptosis research, mitochondrial biology, and cancer cell signaling.

Structurally, Bcl-2-like protein 11 contains a BH3 domain required for binding to anti-apoptotic Bcl-2 members and activating the intrinsic apoptotic pathway. This antibody supports exploration of Bim's role in stress-induced mitochondrial apoptosis and cellular homeostasis.

UniProt: O54918

Pathways: PI3K-Akt Signalweg, Neurotrophin Signalübertragung, Tube Formation, Positive Regulation of Endopeptidase Activity