ME3 Antikörper (AA 17-585)

Produktnummer ABIN7871241

Der Kaninchen Polyklonal anti-ME3 Antikörper wird verwendet zum Nachweis von ME3 in Proben von Human, Maus, Ratte und Affe. Er wurde validiert für WB, ELISA, IHC, IF und FACS.

Kurzübersicht für ME3 Antikörper (AA 17-585) (ABIN7871241)

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ME3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-ME3 Antikörper

Bindungsspezifität: AA 17-585

Verwendungszweck: ME3 Antibody / Malic enzyme 3

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human ME3 recombinant protein (Position: R17-D585) was used as the immunogen for the ME3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the ME3 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the ME3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu ME3

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Andere Bezeichnung: ME3

Hintergrund: ME3 antibody detects Malic enzyme 3, a mitochondrial NADP+-dependent oxidoreductase encoded by the ME3 gene located on chromosome 11q14.2. ME3 belongs to the malic enzyme family, which catalyzes the oxidative decarboxylation of malate to pyruvate, generating NADPH used in lipid biosynthesis and antioxidant defense. ME3 is expressed in brain, kidney, liver, and pancreatic tissues, where it contributes to cellular redox balance and mitochondrial metabolism. The enzyme plays a particularly important role in maintaining NADPH levels required for fatty acid synthesis and glutathione reduction.

Structurally, ME3 is a homotetrameric enzyme localized to the mitochondrial matrix. It shares significant sequence and structural homology with the cytosolic malic enzyme ME1 and the mitochondrial NAD+-dependent isoform ME2 but is distinct in its strict requirement for NADP+ as a cofactor. The protein consists of an N-terminal coenzyme-binding domain and a C-terminal catalytic domain that coordinates divalent metal ions such as magnesium or manganese for catalytic activity. ME3 belongs to the malic enzyme family of oxidative decarboxylases that bridge carbohydrate metabolism with lipid and amino acid biosynthesis.

Functionally, ME3 contributes to metabolic flexibility by linking glycolysis and the tricarboxylic acid (TCA) cycle with biosynthetic pathways. It catalyzes the reversible conversion of malate to pyruvate, generating NADPH for reductive reactions in mitochondria. In pancreatic beta cells, ME3 supports insulin secretion by providing NADPH for redox-sensitive signaling pathways. In neurons, it enhances mitochondrial function and protects against oxidative stress by maintaining NADPH-dependent antioxidant systems such as glutathione reductase. Known substrates include L-malate and NADP+, with pyruvate and carbon dioxide as products.

ME3 activity is closely tied to cellular energy demand and oxidative stress responses. During high biosynthetic activity, ME3-generated NADPH fuels lipid synthesis and reactive oxygen species detoxification. Dysregulation of ME3 expression has been linked to metabolic disorders, neurodegeneration, and cancer. Overexpression can enhance proliferation by increasing NADPH supply for anabolic metabolism, while deficiency impairs mitochondrial redox control. Pathway associations include the TCA cycle, oxidative phosphorylation, and NADPH regeneration pathways. Expression of ME3 increases during metabolic adaptation in tissues such as liver and kidney.

Immunohistochemical staining using ME3 antibody shows mitochondrial localization in hepatocytes, neurons, and renal tubular cells. The ME3 antibody from NSJ Bioreagents is an excellent reagent for studying mitochondrial redox metabolism, energy balance, and biosynthetic regulation.

UniProt: Q16798

Pathways: Warburg Effekt